Динамическая контрастность Улучшенная Магнитно-резонансная томография модели Ортотопическая Рак поджелудочной железы мыши

Overview

This protocol demonstrates the application of dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) for orthotopic pancreatic tumor xenografts in mice. DCE-MRI is a non-invasive imaging technique that allows for the analysis of microvasculature in tumors, providing insights into vascular responses following novel therapies.

Key Study Components

Area of Science

• Neuroscience
• Oncology
• Imaging Techniques

Background

• Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) is used to visualize tumor microvasculature.
• Orthotopic models provide a more accurate representation of the tumor microenvironment compared to subcutaneous models.
• This technique allows for the assessment of neovascularization and tumor cell invasion.
• The study is conducted using human pancreatic cancer cells implanted in mice.

Purpose of Study

• To visualize the microvascular structure of pancreatic tumor xenografts.
• To assess the vascular response of tumors to novel therapies.
• To improve understanding of tumor biology in a more physiologically relevant model.

Methods Used

• Removal of the pancreas from the abdomen.
• Infusion of human pancreatic cancer cells into the tail of the pancreas.
• Placement of a plastic board and surface coil for imaging.
• Utilization of DCE-MRI to visualize the tumor microvasculature.

Main Results

• DCE-MRI successfully visualizes the microvascular structure of the tumor.
• Orthotopic models show enhanced neovascularization compared to subcutaneous models.
• The technique provides insights into tumor blood supply and invasion.
• Demonstrates the effectiveness of DCE-MRI in assessing tumor response to therapies.

Conclusions

• DCE-MRI is a valuable tool for studying pancreatic tumors in vivo.
• Orthotopic models are essential for understanding the tumor microenvironment.
• This method can aid in evaluating the efficacy of new cancer therapies.

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