Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Yishai Yehuda; Britny Blumenfeld; Dan Lehmann; Itamar Simon

doi:10.3791/55157

Журнал

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Генетика

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Генома Определение МЛЕКОПИТАЮЩИХ репликации ДНК с помощью Timing Content измерения

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

JoVE Journal
Генетика

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JoVE Journal Генетика

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Генома Определение МЛЕКОПИТАЮЩИХ репликации ДНК с помощью Timing Content измерения

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8,415 Views

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08:06 min

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January 19, 2017

DOI:

10.3791/55157-v

DOI:

10.3791/55157-v

•

08:06 min

January 19, 2017

•

1 Views

Yishai Yehuda¹, Britny Blumenfeld¹, Dan Lehmann², Itamar Simon¹

¹Dept. of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine,Hebrew University of Jerusalem, ²The Core Research Facility, IMRIC, Faculty of Medicine,Hebrew University of Jerusalem

ТРАНСКРИПТ

Резюме

We describe here a relatively fast and simple approach for mapping genome-wide mammalian replication timing, from cell isolation to the basic analysis of the sequencing results. A genomic map of a representative replication program will be provided following the protocol.