Internal Standards

Source: Laboratory of Dr. B. Jill Venton - University of Virginia

The goal of many chemical analyses is a quantitative analysis, where the amount of a substance in a sample is determined. In order to accurately calculate the concentration of an unknown from a sample, careful sample prepara...

Sample loss can occur every time a sample is handled or transferred, thereby making accurate calculations of concentration difficult.

To ensure accuracy, the effects of sample loss must be minimized using careful sample preparation and by limiting the number of sample handling and transfer steps. However, sample loss can also occur due to ...