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Biomedical Engineering

SEM Sample Prep for Biological Imaging

Description

Scanning electron microscopy (SEM) is used to image biological samples after careful preparation. SEM uses an electron beam in a vacuum to nondestructively view conductive materials and surface features. It works like an optical microscope, but it gives much higher magnification and resolution.

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Transcript

Scanning electron microscopy, or SEM, is often used to image biological materials on the nano scale. Optical microscopes, which use light to image a sample, are heavily used to non-destructively image biological samples, however, their resolution and depth of field is limited, thus SEM is used in order to achieve higher resolution down to one na...

Tags

Scanning Electron MicroscopyBiological Sample PreparationElectron Beam ImagingSample FixationSputter CoatingCritical Point DryingEnvironmental SEMCollagen Hydroxyapatite ScaffoldSecondary Electron Detection