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HIGH SCHOOL

Biology

Science Experiments

Basic Biology

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Biology I: yeast, <em>Drosophila</em> and <em>C. elegans</em>

Yeast Transformation and Cloning

Description

S. cerevisiae are unicellular eukaryotes that are a commonly-used model organism in biological research. In the course of their work, yeast researchers rely upon the fundamental technique of transformation (the uptake of foreign DNA by the cell) to control gene expression, induce genetic deletions, express recombinant proteins, and labe...

Transcript

Yeast or Saccharomyces cerevisiae, is a widespread simple eukaryotic model organism used in the study of genetics and cell biology that can give insights into human cellular processes. This video discusses transformation - the uptake of foreign DNA by the yeast cell. It will introduce yeast plasmids, how to prepare y...

Tags

Yeast TransformationPlasmid PreparationLithium Acetate MethodYeast PlasmidsSelectable MarkerCompetent CellsHeat Shock ProcedureYeast Two HybridFlow CytometryFluorescent Microscopy

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