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HIGH SCHOOL

Biology

Science Experiments

Basic Biology

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Biology I: yeast, <em>Drosophila</em> and <em>C. elegans</em>

Yeast Nucleic Acid Extraction for Lab Use

Description

Yeast nucleic acid extraction makes it possible to collect DNA and RNA from cultured cells. Yeast is a useful model system because it can produce large amounts of biomacromolecules, including nucleic acids.

The process starts with growing and harvesting the yeast cells. The cells are then lysed, ...

Transcript

Today we will be showing you how to purify nucleic acids from Saccharomyces cerevisiae, also known as baker’s yeast. Nucleic acids can include DNA or RNA. This video will discuss the isolation of these molecules from yeast cells by phase separation and chromatography.

Though many differ...

Tags

Nucleic Acid ExtractionYeast Cell LysisColumn BindingPhase SeparationSilica ColumnSpheroplast FormationGlass Bead HomogenizationMicroarray AnalysisGene Expression QuantificationRecombinant Protein Purification

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