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HIGH SCHOOL

Biology

Science Experiments

Basic Biology

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Biology I: yeast, <em>Drosophila</em> and <em>C. elegans</em>

Larval Protein Staining in Drosophila

Description

Larval protein staining in Drosophila uses immunohistochemistry to show where proteins are located in tissues. This method relies on antibodies, which are proteins that bind specific epitopes, or small target regions, on the protein of interest. Because Drosophila larvae are easy to process and are transparent, some tissues ...

Transcript

Drosophila melanogaster is a widely studied model organism due to its versatility and facility of use.

Immunohistochemistry performed on Drosophila larval preparations is an invaluable method that can provide information on the presence, location, and the co-localization of proteins.

This vi...

Tags

Drosophila LarvaeImmunohistochemistryTissue DissectionFixation ProtocolBlocking SolutionPrimary AntibodySecondary AntibodyMounting MediumFluorescence MicroscopyWhole Mount Staining

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