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dissection of imaginal discs from 3rd instar drosophila larvae

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

This protocol demonstrates the dissection technique used for isolating imaginal discs from drosophila larvae at the 3rd instar stage. Methods for fixing the tissue after saying and removing the wing, leg, and eye discs are demonstrated directly on a microscope slide for subsequent visualization.

Today I am going to be showing you how to dissect imaginal discs from third instar drosophila larvae. Third instar larval discs can be used for a number of developmental processes. We look at them in our lab to see the process of apoptosis, but you can express various proteins in them and study them that way and see all sorts of developmental processes going on within the discs in order.In order to do that, I'm going to be using several very important tools. First is the picking stick with which we'll take the larvae out of their dishes. Next, very importantly is the dissecting forceps, which need to be extremely sharp in order for the dissection to go forth.Then we'll be using a surgical blade, number 11 surgical blade to remove the discs from the head. And then we'll be placing the discs on a slide with some vector shield as our mounting media. So the first thing we're going to do is collect some third and star wandering, third and star larvae.I'm just using my picking stick here to sort of pry them off the side of the vial. Sometimes it helps if your stick is a little bit wet and then put them into a drop of PBS. Okay, so here we have our third star larvae.Because I'm right hand

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University of California, Irvine (UCI).  This protocol demonstrates the dissection technique used for isolating imaginal discs from drosophila larvae at the 3rd instar stage. Methods for fixing the tissue after saying and removing the wing, leg, and eye discs are demonstrated directly on a microscope slide for subsequent visualization.

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

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