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1. Validation of Lung Metastases Using India Ink Inflation of Tumor-bearing Lungs
NOTE: To validate the luciferase live tumor imaging results, perform India ink inflation of tumor-bearing mouse lungs to quantify tumor nodules. 4T1 cells also metastasize to other organs and tissues (Figure 1) and therefore, histological examination of these organs to validate tumor metastasis should be performed if needed. This protocol use lung metastasis as a example.
- Keep mice in their existing home cage for euthanasia. When housed with other animals, relocate those mice not being euthanized t....
