Orthotopic Mouse Model
Orthotopic Mouse Model - Related Videos
08:43
Orthotopic Mouse Model of Colorectal Cancer
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Cited by 109 •
2007
Two techniques can be used to establish this model: injection of a cancer cell suspension into the cecal wall or transplantation of a piece of subcutaneous tumor onto the cecum. This model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.
07:01
An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
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Cited by 10 •
2013
Generation of an orthotopic mouse model of anaplastic thyroid carcinoma is described here. This technique employs surgical placement of human anaplastic thyroid cancer cells into the thyroid of immunodeficient mice, thus creating a more clinically relevant setting to study disease progression as well as screen innovative therapeutic interventions.
06:48
Fluorescent Orthotopic Mouse Model of Pancreatic Cancer
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Cited by 5 •
2016
A procedure to implant green fluorescent protein-expressing pancreatic cancer cells (PANC-1 GFP) orthotopically into the pancreas of Balb-c Ola Hsd-Fox1nu mice to assess tumor progression and metastasis is presented here.
05:06
A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis
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Cited by 2 •
2015
The metastatic spread of cancer is the major cause of cancer-related deaths. We provide an in-depth description of our survival surgery methodology for establishing a “patient-like” orthotopic syngeneic mouse model system for studying the mechanisms of metastasis in solid organ tumors.
03:02
Orthotopic Inoculation of Thyroid Cancer Cells into Murine Thyroid: A Procedure to Establish Orthotopic Thyroid Cancer Mouse Model
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2023
This video describes the procedure for orthotopic inoculation of thyroid cancer cells into the thyroid gland of mouse models, which may then serve as a valuable platform to assess carcinogenesis and discover potential treatment strategies.
FAQs
The implantation site does more than determine where a mass appears. It places tumor cells or tissue among local stromal, vascular, and immune components, allowing those elements to interact with the tumor during growth. These interactions can shape invasion, metastatic behavior, and treatment response, making anatomical context an important experimental variable.
An Orthotopic Mouse Model preserves anatomical conditions that are more closely related to the tumor’s site of origin. By contrast, a nonnative site may not reproduce the same local interactions with stromal, vascular, and immune components. This difference can affect observed tumor progression, metastatic behavior, and responses to anticancer treatments.
Local stromal, vascular, and immune components interact with implanted tumor cells or tissue as the tumor develops. Those interactions help create a native-like microenvironment that can influence invasion, metastasis, and treatment response. Including these components gives cancer researchers a way to examine tumor behavior within relevant anatomical and cellular surroundings.
Establishing the model requires implanting tumor cells or tissue into the organ corresponding to the human cancer’s origin. The placement is central to the approach because it creates the intended native-like disease setting. Once established, the model can be used to evaluate tumor progression and responses to anticancer interventions.
Researchers use these models to test anticancer drugs and combination therapies while tumors grow in an anatomically relevant environment. Treatment responses can therefore be examined alongside tumor interactions with local stromal, vascular, and immune components. This helps investigators assess therapeutic effects before clinical studies and compare how interventions influence cancer progression.
These models can support investigation of tumor progression, invasion, metastasis, therapeutic targets, and treatment response. Their value comes from connecting those outcomes to the tumor’s native-like microenvironment rather than examining growth in isolation. In cancer research, that context can inform drug evaluation and the selection of targets for further study before clinical testing.