Papanicolaou Staining: A Technique to Visualize the Cytological Features of Tubal Cells

Abstract

Source: Chen, H. et al. Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection. J. Vis. Exp. (2017).

In this video, we demonstrate a modified protocol of Papanicolaou staining to study tubal cytology. This technique can be used to visualize the cytological features of malignant and non-malignant fallopian tube epithelial cells.

Protocol

1. Modified Papanicolaou Staining Protocol

1. Stain the tissue smeared slides by running the automatized non-gyn stain procedure, as illustrated in Table 1.
   NOTE: The staining method represents a modification of the Papanicolaou staining technique that we use routinely for non-gynecologic specimens. We use an automated tissue processor to stain some slides. We chose the Non-Gyn staining procedure over the Gyn staining procedure in this study. The Eosin used is EA-65, instead of the EA-50 used for gynecological specimens (Pap smear specimen). In addition, OG-6 in the Gyn staining procedure, which is mainly for squa....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Papanicolaou Stain, EA 65	sigma aldrich	HT40432
95% Ethanol	sigma aldrich	652261
100% Ethanol	sigma aldrich	493538
Xylene	sigma aldrich	214736
Hematoxylin	sigma aldrich	H9627