Constructing Patterned Neuronal Circuits on a Multi-Electrode Array

In This Article

Abstract

Source: Kanner, S., et al. Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits. J. Vis. Exp. (2015)

This video demonstrates the process of culturing neuronal cells on a patterned multi-electrode array (MEA) to establish modular neuronal networks. This method allows the study of neural signal transmission and cellular interactions.

Protocol

1. Multi-Electrode Array (MEA) Preparation

Clean MEA in this order (Table of Materials):
1. Wash with water under tap and sonicate in a concentrated, enzymatic detergent 3 times.
2. Sonicate in distilled water 3 times.
3. Wash with distilled water (in hood, 1,000 µl tip) for 3 times and place under ultraviolet (UV) for 30 min.
Supporting network preparation:
1. Prepare the designed support network mold.
  1. Pour polydimethylsiloxane (PDMS) into a 12-well plate (22 mm diameter).
  2. When the PDMS is hardened, remove the mold and, using a scalpe....

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Results

Assembly and microscopy of microfluidic devices; optical analysis setup; microchannel examination.
Figure 1. PDMS structures deposition on a MEA. (A) PDMS mold with a ring shape (marked with the red arrow) mounted on a MEA. The mold is used to coat the area destined for the supporting neuronal network and located at the periphery of the culturing ar.......