Uv Sterilization
Uv Sterilization - Related Videos
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
04:55
UV-Vis Spectroscopy of Dyes - Concepts
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2020
Absorbance When light interacts with a substance, a portion of the light is absorbed while the rest is either reflected or transmitted through it. Substances that we perceive as having a color reflect light in the visible range. The color of the substance that we are able to see depends on which wavelength of light is reflected. A substance that we perceive as blue reflects light in the blue range (430 – 480 nm) of the visible spectrum. According to the color wheel, the same substance absorbs...
09:52
UV-Vis Spectroscopy of Dyes - Student Protocol
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2020
Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA Absorbance Wavelength ComparisonExpand In the first part of the lab, you'll use ultraviolet and visible light absorption spectroscopy, or UV-Vis absorption spectroscopy, to analyze the absorption characteristics of fluorescein, β-carotene, and indigo dye. To perform UV-Vis spectroscopy, you'll place a solution between a light source and a photodetector. The molecules will absorb light at...
09:21
Ultraviolet-Visible (UV-Vis) Spectroscopy
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Ultraviolet-visible (UV-Vis) spectroscopy is one of the most popular analytical techniques because it is very versatile and able to detect nearly every molecule. With UV-Vis spectroscopy, the UV-Vis light is passed through a sample and the transmittance of light by a sample is measured. From the transmittance (T), the absorbance can be calculated as A=-log (T). An absorbance spectrum is obtained that shows the absorbance of a...
01:30
Cleaning, Sterilization, and Disinfection
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2023
Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease. Cleaning The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
FAQs
Germicidal ultraviolet radiation damages microbial DNA and RNA by forming pyrimidine dimers, which are abnormal links between neighboring bases. These lesions interfere with the genetic material’s ability to support replication. As a result, exposed microorganisms may lose the capacity to reproduce and form viable growth, explaining why UV treatment can reduce microbial contamination without adding chemical agents.
The outcome depends on delivering sufficient germicidal radiation to the microorganisms for an adequate period. A low dose or brief exposure may not produce enough damage to prevent viable growth, whereas appropriate dose and exposure time improve microbial reduction. These variables must therefore be considered together when evaluating treatment of a laboratory surface, equipment, air, or water.
UV radiation penetrates poorly, so microorganisms must receive direct exposure for treatment to work effectively. Shadows, enclosed locations, or obstructed surfaces can shield microbes from the radiation even when nearby areas are illuminated. This limitation makes positioning and exposure geometry important, particularly when treating laboratory equipment or surfaces with irregular shapes.
Germicidal UV can injure both skin and eyes, so systems require proper shielding and protective procedures. Safety planning should prevent people from being exposed while radiation is operating and should account for areas where direct UV could reach personnel. These precautions are essential whenever UV treatment is used for laboratory hygiene or biological research spaces.
Applications described for UV sterilization include laboratory surfaces, equipment, air, and water. This range makes the method useful for reducing microbial contamination across different parts of a biological research setting. Its chemical-free nature is particularly relevant to laboratory hygiene, although each target still requires adequate dose, exposure time, and direct line of sight.
A practical application should expose the intended target directly and consider whether any shadows or obstructed regions could remain untreated. Researchers must also select an adequate dose and exposure time, then maintain shielding and protective procedures during operation. These considerations help distinguish effective microbial reduction from apparent treatment in which protected areas retain viable microorganisms.