Generation of Magnetite-Embedded Bacterial Nanocellulose Pellicles

Abstract

Source: Arias, S. L., et al. Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles. J. Vis. Exp. (2016)

This video demonstrates the in situ synthesis of magnetite nanoparticles within a bacterial nanocellulose pellicle produced by Gluconacetobacter xylinus. It explains how magnetic bacterial nanocellulose is generated through iron ion infusion, alkaline precipitation, and purification.

Protocol

1. Synthesis of Polymer-coated Iron Oxide Nanoparticles and Its Deposition in a Bacterial Nanocellulose (BNC) Membrane

1. Bubble 1,000 ml of high-purity water with nitrogen gas in order to remove any dissolved oxygen in the water and replace it with nitrogen.
2. Use a three-neck round-bottom flask to prepare a solution in a 2:1 molar ratio of iron (III) chloride hexahydrate (FeCl₃·6H₂O) and iron (II) chloride tetrahydrate (FeCl₂·4H₂O) diluted with deoxygenated high-purity water. For example, use 5.4 g of FeCl₃·6H₂O and 1.98 g of FeCl₂·4H₂O in 10 ml of d....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Glucoacetobacter xylinus	ATCC	700178
Iron(III) Chloride Hexahydrate	Sigma Aldrich	236489-100G
Ammonium Hydroxide	Macron Fine Chemicals	6665-46
Poly(Ethylene Glycol), Average Mn 400	Sigma Aldrich	202398-250G
Iron (II) chloride tetrahydrate	Sigma Aldrich	44939-250G
Anhydrous Calcium Sulfate	W.A. Hammond Drierite	13001
High vacuum grease	Sigma Aldrich	Z273554-1EA
pH test strips	Sigma Aldrich	P4786-100EA
Round-bottom three neck angle type distilling flask	Sigma-Aldrich	CLS4965250
Silicone oil for oil baths	Sigma-Aldrich	85409-250ML
Drying Tube	Chemglass	CG-1295-01
Septum Stopper, Sleeve Type	Chemglass	CG-3022-98
Magnetic stir bar	Chemglass	CG-2001-05
Condenser	Chemglass	CG-1218-01
Temperature Controller	BriskHeat	SDC120JC-A
Stirring Hotplate	Fisher Scientific	11-100-49SH