An In Vitro Assay to Study Microbial Interactions in Co-Culture

Abstract

Source: Temkin, M. I. et al. High Throughput Co-culture Assays for the Investigation of Microbial Interactions. J. Vis. Exp. (2019)

This video demonstrates an in vitro assay to study microbial interactions between Corynebacterium propinquum and Staphylococcus in a co-culture.

Protocol

1. Target Organism Inoculation

NOTE: After overnight incubation, ensure that the cultures are turbid. Some bacterial cultures may flocculate at the bottom of the culture tube. Vortex the culture tubes to disperse clumps and assess the culture turbidity.

1. Prepare the target plate by filling each well of an empty 12 well plate with 1.8 mL of Brain-heart-infusion (BHI) broth and 200 µL of the target overnight culture.
   NOTE: A target organism refers to the organism whose inhibition status is determined using the co-culture interaction assay. For this experiment, the target organisms are St....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
12-well cell culture plate, sterile with lid	Greiner bio-one	665 180
Agar, bacteriological	VWR	J637
Brain Heart Infusion Broth	Dot Scientific	DSB11000-5000