JoVE Encyclopedia of Experiments
Microbiology
0 views • 3:30 min • February 26th, 2026
Begin with bioassay plates, one pre-colonized on the left side of the nutrient agar with Corynebacterium propinquum and the other containing nutrient agar alone.
Next, take a multi-well plate containing pathogenic Staphylococcus suspension.
Lower an inoculation stamp into the wells, with its tips dipping into the Staphylococcus culture.
Swirl the stamp, then lift it and verify that each tip carries a uniform droplet to ensure consistent inoculation.
Align the stamp with the right side of the bioassay plate, avoiding contact with the existing colonies.
Remove the stamp, leaving Staphylococcus inoculum spots on the agar.
Repeat the stamping on the agar alone plate to generate a monoculture. Incubate the plates upside down.
In the coculture, C. propinquum secretes siderophores, small molecules that scavenge iron from the agar, thereby inhibiting Staphylococcus growth.
Post-incubation, compare Staphylococcus growth in the co-culture and the monoculture.
Significant inhibition in the co-culture suggests interaction between the two species.
After the six-day incubation of the bioassay plate, prepare overnight cultures of the target organism as previously described. Prepare the target plate by fill
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