Mouse Echocardiography: A Non-invasive Technique to Assess Cardiac Morphology

Abstract

Source: Baudouy, D., et al. Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse. J. Vis. Exp. (2017).

This video describes a protocol to perform echocardiography on a laboratory mouse.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Echocardiography

1. Determine the body weight of the mouse using a standard laboratory balance while holding it lightly by the tail to ensure proper positioning.
2. Anesthetize the animal by the intraperitoneal (i.p.) injection of 50 mg/kg pentobarbital.
   NOTE: Any other kind of anesthesia can be used if the same protocol is used throughout the study. Advantages and disadvantages will be discussed below.
3. Put the mouse back in its ....

Results

Figure 1: Standard-resolution echocardiography (>10 MHz) to measure LV systolic and diastolic dimensions and cardiac function in mice with dilated cardiac hypertrophy after myocardial infarction versus control animals. Representati.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Ultrasound transmission gel; Gel Aquasonic 100	Parker
Linear ultrasound probe; L15-7io	Philips Healthcare
Echocardiograph; IE33 xMATRIX	Philips Healthcare
Rodent shaver	Harvard Apparatus	34-0243