Colonoscopic Guided Murine Wound Model: A Method to Isolate Biopsy Induced Wound Bed from Colon to Understand the Healing Process

Abstract

Source: Montrose, D. C., et al. Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes. J. Vis. Exp. (2021).

In this video, we describe a procedure to isolate the colonoscopic-induced wound bed at an appropriate time point for detailed molecular analysis. This analysis helps to understand the wound healing process in inflammatory conditions of the GI tract.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Collection of the wound bed for molecular analysis

1. Euthanize mouse using CO₂ asphyxiation followed by cervical dislocation (or equivalent technique) on the selected day following biopsy.
2. Harvest the distal region of the colon by opening the skin and abdominal muscle to expose the body cavity. Place closed scissors under the colon and gently lift to release it from the underlying mesentery and then cut the colon at its midpoint and at the anus to remove it from th....

Results

Figure 1: Ex vivo and sectioned images of wound bed. (A) A colon was harvested from a mouse 2 days following biopsy, stained with 0.2% methylene blue and imaged under a dissecting microscope. The dashed circle indicates the edges of the wound bed. The black line .......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Biopsy forceps, 3 Fr	Karl Storz	61071ZJ
isofluorane	Covetrus	2905
methylene blue	Sigma-Aldrich	M9140
micro iris scissors	Integra	18-1619
scalpal with #10 blade	Hill-Rom	372610