Constructing Patterned Neuronal Circuits on a Multi-Electrode Array

Abstract

Source: Kanner, S., et al. Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits. J. Vis. Exp. (2015)

This video demonstrates the process of culturing neuronal cells on a patterned multi-electrode array (MEA) to establish modular neuronal networks. This method allows the study of neural signal transmission and cellular interactions.

Protocol

1. Multi-Electrode Array (MEA) Preparation

1. Clean MEA in this order (Table of Materials):
   1. Wash with water under tap and sonicate in a concentrated, enzymatic detergent 3 times.
   2. Sonicate in distilled water 3 times.
   3. Wash with distilled water (in hood, 1,000 µl tip) for 3 times and place under ultraviolet (UV) for 30 min.
2. Supporting network preparation:
   1. Prepare the designed support network mold.
      1. Pour polydimethylsiloxane (PDMS) into a 12-well plate (22 mm diameter).
      2. When the PDMS is hardened, remove the mold and, using a scalpe....

Results

Figure 1. PDMS structures deposition on a MEA. (A) PDMS mold with a ring shape (marked with the red arrow) mounted on a MEA. The mold is used to coat the area destined for the supporting neuronal network and located at the periphery of the culturing ar.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
PDMS, Sylgard 184	Dow Corning
Nalgene Vacuum Chamber	Thermo	5305-0609
Poly-D-Lysine PDL	Sigma	P7886
12 well culture plate	Sigma	CLS3336
5-Fluoro-2'-deoxyuridine	Sigma	F0503
MEA1060-Inv-BC	Multi Channel Systems
TC02	Multi Channel Systems
Pen Strep	Biological Industries Beit Haemek	03-033-1c
B-27	Gibco	17504044
glutaMAX	Gibco	35050-038
MEM Minimum Essential Medium-Eagle	Biological Industries Beit Haemek	01-025-1B
Micro Electrode Arrays 4Q	Multi Channel Systems	60-4QMEA1000iR-Ti-pr	cleaning manual: http://www.multichannelsystems.com