Subdural Implantation of a Soft Electrocorticography Array for Cortical Electrophysiology Recording in Minipigs

Abstract

Source: Fallegger, F. et al., Subdural Soft Electrocorticography (ECoG) Array Implantation and Long-Term Cortical Recording in Minipigs. J. Vis. Exp. (2023).

This video demonstrates the implantation of a soft electrocorticography array and the long-term cortical recordings in a minipig. It outlines the steps involved in surgically implanting the array over the minipig's auditory cortex and recording both baseline and sound stimulus-evoked neuronal action potentials.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Surgical implantation of soft electrocorticography (ECoG) arrays

1. Anesthesia
   1. Premedication: Isolate the Female Göttingen minipigs (n = 7) at 2-6 months of age (5-8 kg) and fast it overnight. Inject a mix of midazolam at 0.75 mg/kg, atropine at 0.25 µg/kg, and haldol at 0.1 mg/kg intradermally, and wait until the animal is sedated. Weigh the animal before proceeding.
   2. Installation of intravenous (IV) lead:
      1. Place the animal ....

Results

Figure 1: Minimally invasive implantation of soft ECoG onto the brain. (A) Surgical access to the skull, with indication of bregma. (B) Bilateral craniotomy with visible dura mater. (C) Slit durotomy.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Bone drill	BBraun	Elan 4 with GA861 handpiece
Bone drill bit	BBraun	Neurocutter GP204R
Bonewax	Ethicon	W31G
Catheter	Venisystems	Abbocath 14G
Gelfoam	Pfizer	Gelfoam
Insert speakers	Etymotic	Etymotic ER2 insert Earphones
Potentiostat	Gamry Instruments	Reference 600
Pulse Generator	AM Systems	Model 2100 Isolated Pulse Stimulator
Recording headstage	Multichannel systems	W2100-HS32
Recording system	Multichannel systems	W2100
Screwdriver	Medtronic	Handle: 001201, Shaft: 8001205
Stab knife	Fine Science Tools	10316-14
Suture wire dermal	Ethicon	Vicryl 2-0
Suture wire dura mater	Ethicon	Mersilk 5-0
Suture wire for lifting dura	Ethicon	Prolene 6-0 with BV-1 needle
Suture wire subcutaneous	Ethicon	Vicryl 4-0
Titanium bridge	Medtronic	TiMesh 015-2001-4	Cut out the required size
Titanium screws	Medtronic	9001635, 9001640
X-ray system	GE	GE OEC 9800 Plus C-Arm