Targeted Substance Delivery to the Mouse Brain via Infusion into the Internal Carotid Artery

Abstract

Source: Leda, A. R., et al. Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery. J. Vis. Exp. (2017)

This video demonstrates a microsurgical procedure designed to facilitate the targeted infusion of a test substance into the brain via the internal carotid artery (ICA). The procedure involves precise suturing and vascular manipulation to temporarily restrict blood flow, ensuring controlled delivery. A capillary tube is inserted into the external carotid artery (ECA) and advanced toward the bifurcation. Upon infusion, the substance bypasses systemic circulation, crosses the blood-brain barrier, and achieves targeted delivery to the brain parenchyma.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of Mice for Surgery

1. Anesthetize mouse with isoflurane mixed with oxygen, using a laboratory anesthesia system. Use isoflurane at setting between 4-5% and oxygen flow at 2 L/min on the commercial machine (See Materials Table). Transfer the animal to the surgery surface, under a stereomicroscope, and sustain anesthesia using a nose cone (use isoflurane setting 1.5 - 2.5 and oxygen flow at 2 L/min).
   1. Ensure that the mouse respiratory rate is around 1 - 2 respira....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Anesthesia instrument	Vetequip	901806
Surgical scissors	Fine Science Tool	14558-09
Surgical forceps straight tip	Fine Science Tool	00108-11
Surgical forceps angled tip	Fine Science Tool	00109-11
Spring scissors	Fine Science Tool	15000-08
Nylon suture	Braintree Scientific	SUT-S 104
Capillary tubing (Micro-Renathane 0.010” x 0.005” per ft.)	Braintree Scientific	MRE01050
Closing suture	VWR	95057-036
Isoflurane	Piramal
2,3,5-Triphenyltetrazolium chloride	FisherScientific	50-121-8005