JoVE Encyclopedia of Experiments
Neuroscience
0 views • 3:42 min • June 17th, 2025
Take an anesthetized mouse with an exposed common carotid artery or CCA, which bifurcates into the external carotid artery or ECA and the internal carotid artery or ICA.
Tightly suture the distal ECA, place a loose knot near the bifurcation, then clip the CCA and ICA to restrict blood flow.
Incise the ECA, then insert a capillary connected to a syringe containing the test substance.
Advance the capillary between the bifurcation and the CCA clip, then secure it by tightening the proximal ECA knot.
Remove the ICA clip and infuse the substance for targeted delivery to the brain while bypassing systemic circulation.
The substance crosses the blood-brain barrier to reach the brain parenchyma.
Once the ECA and ICA are exposed, use forceps to place a segment of 1 centimeter nylon suture under the external carotid artery, then place a second 1 centimeter segment. Then tie a permanent knot at the highest point possible of the ECA.
At the lowest point possible of the ECA immediately above the CCA bifurcation, tie a looser removable knot. Then use vessel clips to close the CCA at the lowest possible point and to close the ICA.
Properly closing the internal carotid artery before making a small cut in
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