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pdms device fabrication and surface modification

PDMS Device Fabrication and Surface Modification

My name is Ken Kotz. I am a postdoc here at the Bio MAs Resource Center affiliated with Massachusetts General Hospital. My main research project involves isolating neutrophils using an immuno affinity capture device with microfluidic structures.We use this device at five different hospitals across the country to isolate RNA for downstream genomic analysis. Basically what you have is a master of SU eight photo resist on top of silicon. We're going to pour a two part elastomer A-P-D-M-S on top of that master and that PDMS will form a mold.We remove the master mold from its case and place it into a Petri dish. The master is secured by tape on the bottom of a Petri dish. On the scale we weigh out a mixture of of PDMS, hardener and base resin.The ratio is one part hardener to 10 parts resin. Typically, for our devices that have been cut out, we weigh out 20 to 30 grams of the resin and two to three grams respectively of the hardener. After pouring both the hardener and the resin into the small whey boat, we use a standard plastic fork to mix the two together.You wanna make sure that you mix vigorously and fold the mixture on top of one another to ensure that the hardener is evenly distr

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MGH - Massachusetts General Hospital.  PDMS Device Fabrication and Surface Modification

PDMS Device Fabrication and Surface Modification

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