Bioluminescence Imaging of an Immunocompetent Mouse Model for Glioblastoma

Abstract

Source: Clark, A.J., et al. Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma. J. Vis. Exp. (2016)

This video demonstrates bioluminescence imaging to track glioblastoma progression in an immunocompetent mouse model, using luciferase-expressing tumor cells and luciferin to quantify tumor growth non-invasively.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Bioluminescence Imaging of GL261 Tumor Growth

1. Spray a generous amount of disinfectant (0.05% dimethyl ammonium chloride solution) on a clean paper towel. Use the disinfectant-soaked paper towel to thoroughly wipe the black sheet, nose cones, and divider inside the imaging chamber where the animals will be in contact with the device. Thoroughly wipe all surfaces with a clean, dry paper towel. Repeat this once more and wait 5 min.
2. Initialize the imaging station.
3. Anesthetize the mouse with a 300 &....

Results

Figure 1. Luciferase expression does not affect in vivo tumor growth and animal survival. (A) Bioluminescence .......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
D-Luciferin, Potassium salt	Gold Biotechonology	LUCK-100	Store away from light
Living Image software	Caliper Life Sciences	Contact for Quote	None
Xenogen Lumina	Caliper Life Sciences	Contact for Quote	None
Ketaset	Pfizer	NDC 0856-2013-01	Control substance
Xylazine	Sigma-Aldrich	23076-35-9	None
28 G needle (with syringe)	Fisher	22-004-270	AKA Insulin Syringe
Heating pad	Dunlap	HP950
PDI Alcohol prep pads	Fisher	23-501-711