Erratum: Generation of Transgenic C. elegans by Biolistic Transformation

Summary

The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect.

Abstract

The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect.

Protocol

The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect. The volume has been corrected from:

The spermidine is unstable in aqueous solution. We make 70μl spermidine aliquots and store at -20°C. We then add 1mL of water right before use to obtain a 0.1M solution. This solution must be prepared fresh for each bombardment.

to:

The spermidine is unstable in aqueous solution. We make 16μl spermidine aliquots and store at -20°C. We then add 1mL of water right before use to obtain a 0.1M solution. This solution must be prepared fresh for each bombardment.