Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters

Summary

Here, we describe a protocol for synthesis of magneto-plasmonic nanoparticles with a strong magnetic moment and a strong near-infrared (NIR) absorbance. The protocol also includes antibody conjugation to the nanoparticles through the Fc moiety for various biomedical applications which require molecular specific targeting.

Abstract

Magnetic and plasmonic properties combined in a single nanoparticle provide a synergy that is advantageous in a number of biomedical applications including contrast enhancement in novel magnetomotive imaging modalities, simultaneous capture and detection of circulating tumor cells (CTCs), and multimodal molecular imaging combined with photothermal therapy of cancer cells. These applications have stimulated significant interest in development of protocols for synthesis of magneto-plasmonic nanoparticles with optical absorbance in the near-infrared (NIR) region and a strong magnetic moment. Here, we present a novel protocol for synthesis of such hybrid nanoparticles that is based on an oil-in-water microemulsion method. The unique feature of the protocol described herein is synthesis of magneto-plasmonic nanoparticles of various sizes from primary blocks which also have magneto-plasmonic characteristics. This approach yields nanoparticles with a high density of magnetic and plasmonic functionalities which are uniformly distributed throughout the nanoparticle volume. The hybrid nanoparticles can be easily functionalized by attaching antibodies through the Fc moiety leaving the Fab portion that is responsible for antigen binding available for targeting.

Introduction

Hybrid nanoparticles consisting of different materials with distinct physicochemical properties can open new opportunities in biomedical applications including multimodal molecular imaging, therapy delivery and monitoring, new screening and diagnostic assays^1-3. The combination of plasmonic and magnetic properties in a single nanoparticle is of particular interest because it provides a very strong light scattering and absorption cross-sections associated with plasmon resonances and responsiveness to a magnetic field. For example, magneto-plasmonic nanoparticles were used to increase contrast in dark-field imaging of labeled cells by applying a temporal signal modulation via an external electromagnet^3-5. More recently, a similar principle was applied in development of a new imaging modality – magneto-photoacoustic imaging, where magneto-plasmonic nanoparticles enable great improvements in contrast and signal-to-background ratio^6,7. It was also shown that the hybrid nanoparticles can be used for simultaneous capture and detection of circulating tumor cells in whole blood and in vivo^8,9. Furthermore, magneto-plasmonic nanoparticles are promising theranostic agents which can be used for molecular specific optical and MR imaging combined with photothermal therapy of cancer cells¹⁰.

Several approaches were explored for synthesis of magneto-plasmonic nanoparticles. For example, Yu et al. utilized decomposition and oxidation of Fe(CO)₅ on gold nanoparticles to form dumbbell-like bifunctional Au–Fe₃O₄ nanoparticles¹¹. Wang et al. have synthesized gold-coated iron oxide nanoparticle by using thermal decomposition method¹². Some other approaches rely on coating polymer or amine functional molecules onto magnetic core nanoparticles followed by deposition of a gold shell onto the polymer surface to create the hybrid particles^7,13. In addition, iron-oxide nanoparticles were attached to gold nanorods via electrostatic interactions or a chemical reaction^14,15. Although these approaches yield magneto-plasmonic nanostructures, they compromise to some extent properties of the magneto-plasmonic combination such as optical absorbance in the near-infrared (NIR) window or a strong magnetic moment both of which are highly desirable in biomedical applications. For example, dumbbell Au-Fe₃O₄ nanoparticles have a plasmon resonance peak at 520 nm which limits their utility in vivo due to high tissue turbidity in this spectral range. Furthermore, the magneto-plasmonic nanoparticles produced by current protocols are limited to just one¹¹ or few (less than 10)^14,15 superparamagnetic moieties (e.g., iron oxide nanoparticles) that is significantly less than could be achieved in a densely packed nanostructure. For example, a densely packed 60 nm diameter spherical nanoparticle can contain on the order of a thousand of 6 nm superparamagnetic nanoparticles. Therefore, there is a great room for improving magnetic properties of the hybrid nanoparticles. Moreover, some of the previously described protocols are relatively complex and require careful optimization in order to avoid particle aggregation during synthesis^14,15.

Here, we describe a protocol for synthesis of magneto-plasmonic nanoparticles with a strong magnetic moment and a strong NIR absorbance that addresses major limitations of the current art. The synthesis has its origins in oil-in-water microemulsion method¹⁶. It is based on assembly of nanoparticles of a desired size from a much smaller primary particles. This approach has been successfully used to produce nanostructures from a single material such as gold, iron oxide, and semiconductor primary particles¹⁶. We extended it to synthesis of magneto-plasmonic nanoparticles by, first, making 6 nm diameter gold shell/iron oxide core particles and, then, assembling the primary hybrid particles into the final spherical nanostructure. Assembling primary particles into nanoclusters not only allows enhancing the properties of constituent nanoparticles, such as achieving a stronger magnetic moment while preserving superparamagnetic properties, but also takes advantage of the interactions between individual nanoparticles thus creating new characteristics absent from the constituent nanoparticles, such as strong optical absorbance in the NIR window. This protocol yields hybrid nanoparticles with a high density of magnetic and plasmonic functionalities. After primary particles are synthetized, our method is essentially a simple one-pot reaction. The overall plasmon resonance strength and magnetic moment are determined by a number of primary particles and, therefore, can be easily optimized depending on an application. Furthermore, we also developed a procedure for antibody conjugation to the hybrid nanoparticles for various biomedical applications which require molecular specific targeting. Antibodies are attached through the Fc moiety leaving the Fab portion that is responsible for antigen binding available for targeting.

Protocol

1. Instrumentations and Glassware Preparation

1. Wear appropriate protective equipment, i.e., a lab coat, disposable gloves, and eye protection.
2. Connect a round-bottom flask to a condenser and immerse it in a silicone oil bath with a temperature monitoring by a thermometer. Place a source of heat (e.g., hot plate) under the oil bath (Figure 1). Use a thermometer capable of measuring the temperature higher than 260 °C.

2. Synthesis of Primary Hybrid Magneto-plasmonic Nanoparticles

1. Making Magnetic Core Nanoparticles
   1. Add 353.2 mg (1 mmol) iron(III) acetylacetonate, 1 ml (2 mmol) oleic acid, 1 ml (2 mmol) oleylamine, 1.292 g (5 mmol) 1,2-hexadecanediol, and 10 ml phenyl ether to a round-bottom flask.
   2. Stir the mixture vigorously using a magnetic stir-bar and heat to 250-260 °C for 1 hr under reflux. Then, wait for the solution to cool down to RT. Ensure the temperature is under 260 °C to prevent boiling of the phenyl ether and prevent a burst of the reaction mixture from the round-bottom flask to the condenser.
      CAUTION: The reaction mixture is extremely hot and the chemicals may cause irritation. Must operate under a fume hood and wear appropriate personal protection equipment. Ensure adequate ventilation for the oil bath.
      NOTE: The oil bath is kept at 250-260 ºC temperature for 1 hr during synthesis of the magnetic nanoparticles. In principle, a Pyrex glass dish can be used for this purpose. However, the maximum continuous temperature for Pyrex glass is ~260 ºC according to vendor information. Therefore, a metal container provides a safer option for the reaction as it can withstand a higher temperature and last longer during multiple runs.
2. Deposition a gold shell onto magnetic core nanoparticles
   1. Add 411.5 mg (1.1 mmol) gold acetate, 0.25 ml (0.75 mmol) oleic acid, 1.5 ml (3.0 mmol) oleylamine, 775.3 mg (3 mmol) 1,2-hexadecanediol, and 15 ml phenyl ether to a round-bottom flask.
   2. Add 5 ml suspension of magnetic nanoparticles from step 2.1. Heat the reaction mixture to 180 °C and keep under reflux for 1 hr. Wait for the solution to cool down to RT.
   3. Add 50 ml ethanol to precipitate the hybrid primary nanoparticles followed by centrifugation at 3,250 × g for 15 min.
   4. Resuspend the precipitate in 25 ml hexane by using a bath sonicator. Add 25 ml ethanol to precipitate the primary hybrid nanoparticles. Centrifuge at 3,250 x g for 15 min and resuspend the precipitate in hexane. Repeat this step three times.
   5. Dry the precipitated primary hybrid nanoparticles in a vacuum desiccator O/N. Confirm that the particles are completely dry.

3. Hybrid Magneto-plasmonic Nanoclusters Synthesis and Size Separation

1. Resuspend 5 mg of dry primary hybrid nanoparticles in 1 ml hexane by using a bath sonicator. Sonicate the nanoparticle suspension until no visible precipitate is present.
2. Add the solution from step 3.1 to 10 ml aqueous solution of sodium dodecyl sulfate (2.8 mg/ml) in a 20 ml glass vial with attached caps. Add the suspension of primary hybrid nanoparticles drop by drop to avoid mixing of the two phases before the next step.
3. Sonicate the two-phase solution in a bath sonicator for 2 hr, followed by heating in a water bath at 80 °C for 10 min. Wait for the solution to cool down to RT.
   1. Fill water to the operating level line of the sonication bath. Center the glass vial in the sonication bath. An emulsion forms immediately between the two phases. Shake the two-phase solution by hand after beginning of the sonication; this facilitates mixing between the phase containing primary hybrid nanoparticles and the bottom aqueous phase.
      NOTE: Be aware that the sonicator will heat up after 2 hr of operation.
4. Centrifuge the hybrid nanocluster suspension at 100 x g for 30 min. Collect both the precipitate and the supernatant. Resuspend the precipitate in 0.1 mM sodium citrate under 10 min sonication. The expected size of the nanoclusters is ~180 nm in diameter.
5. Transfer the supernatant from step 3.3 to a new conical tube.
6. Centrifuge the suspension from step 3.4 at 400 x g for 30 min. Collect both the precipitate and the supernatant. Resuspend the precipitate in 0.1 mM sodium citrate under 10 min sonication. The expected size of the nanoclusters is ~130 nm in diameter.
7. Transfer the supernatant from step 3.5 to a new conical tube.
8. Centrifuge the suspension from step 3.6 at 1,500 x g for 30 min. Collect the precipitate and resuspend in 0.1 mM sodium citrate under 10 min sonication. The expected size of the nanoclusters is ~90 nm in diameter.
9. Add 300 μl nanocluster suspension to a 96-well microplate reader for measuring a UV-Vis-NIR absorption spectrum. Drop 10 μl nanocluster suspension onto carbon-coated copper grid for TEM imaging.

4. Conjugation of Monoclonal Antibodies to Nanoclusters

1. Prepare 100 μl monoclonal antibody solution (1 mg/ml) in PBS, pH 7.2, e.g., anti-Epidermal Growth Factor Receptor 2 (HER2) antibodies or anti-Epidermal Growth Factor Receptor 1 (EGFR) antibodies.
2. Add the antibody solution from step 4.1 to 3.9 ml 4 mM HEPES, pH 7.2. Centrifuge the solution through a 10 k MWCO centrifugal filter at 3,250 x g for 20 min at 8 °C. Resuspend the antibody in 4 mM HEPES, pH 7.2, to a final volume of 100 μl.
   NOTE: This step is carried out to replace the original media in the antibody solution with HEPES.
3. Add 10 μl of 100 mM NaIO₄ to 100 μl of antibody solution. Cover the reaction vial with an aluminum foil at RT and mix for 30 min using an orbital shaker.
4. Quench the reaction by adding 500 μl of 1x PBS.
5. Add 2 μl of 46.5 mM linker solution (dithiolaromatic PEG6-CONHNH₂) to the antibody solution from step 4.4 and shake for 1 hr at RT.
6. Filter the solution using a 10 k MWCO centrifuge filter at 3,250 x g for 20 min at 8 °C. Resuspend the antibody in 1x PBS to a final volume of 100 μl which leads to an antibody concentration of ~1 mg/ml.
7. Mix 100 μl nanocluster suspension at O.D. ~1.0 with 1 μl modified antibodies from step 4.6 (1 mg/ml) for 120 min at RT.
8. Add 10 μl of 10^-3 M 5 kDa thiol PEG and shake for 15 min at RT.
9. Centrifuge the solution at 830 x g for 3 min. Discard the supernatant and resuspend the sediment in 100 μl 2% w/v 5 kDa PEG in PBS, pH 7.2.
10. Measure the absorbance spectrum of the antibody-conjugated nanoclusters and compare to the absorbance spectrum of the bare nanoclusters. Expect a couple of nanometer red shift after the conjugation.
11. If the nanoparticles aggregate as shown by a significant shift with an increase in O.D. in the red-NIR region, increase the concentration of thiol PEG to 5 x 10^-3 M. Also, increase the incubation time with thiol PEG to 30 min and decrease the centrifugal speed in 200 x g increments.
12. For cancer cell labeling test, add the antibody conjugated nanoparticles from step 4.9 to cancer cell suspension in either medium or 1x PBS (1 ml ~10⁶ cells) and mix for 60 min.

Results

A scheme for synthesis of immunotargeted magneto-plasmonic nanoclusters is shown in Figure 2. First, magnetic Fe₃O₄ iron oxide nanoparticles are synthetized via thermal decomposition method. Then, a thin ca. 1 nm gold shell is deposited on the iron oxide core particles via thermal decomposition. The primary ca. 6 nm hybrid nanoparticles serve as seeds to create magneto-plasmonic nanoclusters by utilizing an oil-in-water microemulsion approach. The nanoclusters are ...

Discussion

Critical steps in successful synthesis of magneto-plasmonic nanoclusters include making highly monodispersed primary gold shell/iron oxide core nanoparticles and directing self-assembly of the primary particles into nanoclusters. A molar ratio between the primary particles and surfactants play an important role in determining size distribution of the nanoclusters. Non-uniform size distribution of primary nanoparticles may cause formation of big aggregates during assembly of magneto-plasmonic nanoclusters. In addition, th...

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
PYREX 50 ml round bottom boiling flask with short neck & 24/40 [ST] joint	Corning	4320A-50	Thermal decomposition reaction
PYREX 41 x 300 mm 5-bulb Allihn condenser with 24/40 [ST] outer/inner joints	Corning	2480-300	Thermal decomposition reaction
Silicone oil	Fisher	S159-500	Oil bath
Hot plate stirrer	Corning	PC-351	Heat the reacton with stirring function
Thermometer	ThermoWorks	221-092	Measure temperature
Iron(III) acetylacetonate	Fisher	AC11913-0250	Material for primary hybrid nanoparticles synthesis
Oleic acid 99%	Fisher	A195-500	Material for primary hybrid nanoparticles synthesis
Gold(III) acetate	Fisher	AA3974206	Material for primary hybrid nanoparticles synthesis
Hexane	Fisher	H292-1	Material for primary hybrid nanoparticles synthesis
Phenyl ether 99%	Fisher	AC13060-0025	Material for primary hybrid nanoparticles synthesis
1,2-Hexadecanediol 90%	Sigma	213748-50G	Material for primary hybrid nanoparticles synthesis
Oleylamine 70%	Sigma	O7805-100G	Material for primary hybrid nanoparticles synthesis
Sodium dodecyl sulfate	Fisher	BP166-100	Cluster synthesis
Sodium citrate dihydrate	Sigma	W302600	Cluster synthesis
Monoclonal anti-EGF receptor antibody	Sigma	E2156	Cell labeling specificity test
Monoclonal anti-HER2 antibody	Sigma	AMAB90627	Cell labeling specificity test
Sodium periodate	Sigma	311448	Oxidate Fc region of antibodies
Dithiolaromatic PEG6-CONHNH2	SensoPath Technologies	SPT-0014B	Heterofunctional linker for antibody conjugation to nanoclusters
Methoxy-PEG-thiol, 5 k	Creative PEGworks	PLS-604	Passivate the remaining gold surface after antibody conjugation
Amicon Ultra-4 centrifugal filter unit with Ultracel-10 membrane	Millipore	UFC801008	Protein purification
HEPES	Sigma	H3375	Buffer
PBS, 1x solution	Fisher	BP2438-20	Buffer
UV-Vis spectroscopy	BioTek	Synergy HT	Obtain spectrum
Centrifuge	Eppendorf	5810R	Separation
Transmission Electron Microscope	FEI	TECNAI G2 F20 X-TWIN	Obtain morphology of nanostructures
Upright microscope	Leica	DM6000	Obtain dark-field images
Sonicator	Branson	1510	Sonication
Carbon film 300 mesh grid	EMS	CF300-Cu	TEM imaging
96-well plate	Corning	09-761-145	UV-Vis reading plate