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Method Article

Preparation of Carbon Nanosheets at Room Temperature

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DOI:

10.3791/53505

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March 8th, 2016

 ,  ,  ,  ,  , 
1Institute of Materials, Ecole Polytechnique Fédérale de Lausanne (EPFL), 2Colloid Chemistry Department, Max Planck Institute of Colloids and Interfaces

In This Article

Summary

We present the synthesis of an amphiphilic hexayne and its use in the preparation of carbon nanosheets at the air-water interface from a self-assembled monolayer of these reactive, carbon-rich molecular precursors.

Abstract

Amphiphilic molecules equipped with a reactive, carbon-rich "oligoyne" segment consisting of conjugated carbon-carbon triple bonds self-assemble into defined aggregates in aqueous media and at the air-water interface. In the aggregated state, the oligoynes can then be carbonized under mild conditions while preserving the morphology and the embedded chemical functionalization. This novel approach provides direct access to functionalized carbon nanomaterials. In this article, we present a synthetic approach that allows us to prepare hexayne carboxylate amphiphiles as carbon-rich siblings of typical fatty acid esters through a series of repeated bromination and Negishi-type cross-coupling reactions. The obtained compounds are designed to self-assemble into monolayers at the air-water interface, and we show how this can be achieved in a Langmuir trough. Thus, compression of the molecules at the air-water interface triggers the film formation and leads to a densely packed layer of the molecules. The complete carbonization of the films at the air-water interface is then accomplished by cross-linking of the hexayne layer at room temperature, using UV irradiation as a mild external stimulus. The changes in the layer during this process can be monitored with the help of infrared reflection-absorption spectroscopy and Brewster angle microscopy. Moreover, a transfer of the carbonized films onto solid substrates by the Langmuir-Blodgett technique has enabled us to prove that they were carbon nanosheets with lateral dimensions on the order of centimeters.

Introduction

Two-dimensional carbon nanostructures attract significant attention due to the reported outstanding electrical, thermal, as well as mechanical properties1-5. These materials are expected to further the technical progress in the fields of polymer composites6, energy storage devices7, and molecular electronics8-10. Despite intensive research efforts in recent years, however, access to larger amounts of well-defined carbon nanomaterials is still limited, which impedes their large-scale implementation in technological applications11,12.

Carbon nanomaterials are accessible by either top-down or bottom-up approaches. Typical approaches such as exfoliation techniques13 or high-energy processes on surfaces14-16 offer the possibility to obtain materials with a high degree of structural perfection and very good performance. However, the isolation and purification of the products remains challenging, and the large-scale production of defined nanostructured materials is difficult12. On the other hand, bottom-up approaches can be employed that rely on the use of molecular precursors, their arrangement into defined structures, and a subsequent carbonization that yields the carbon nanostructures17-23. In this case, the precursors themselves are more complex and their preparation often requires multiple synthetic steps. These approaches may offer a high degree of control over the chemical and physical properties of the resulting materials and may furnish a direct access to tailored materials. However, the conversion of the precursors into carbon nanomaterials is typically performed at temperatures above 800 °C, which leads to a loss of the embedded chemical functionalization24-27.

The above mentioned limitations have been addressed in our group by employing highly reactive oligoynes that can be converted into carbon nanomaterials at room temperature28,29. In particular, amphiphiles comprising a hydrophilic head group and a hexayne segment are accessible through a sequence of bromination and palladium-mediated Negishi cross-coupling reactions30,31. The conversion of these precursor molecules into the target structure occurs at or below room temperature upon irradiation with UV light. The high reactivity of the oligoyne amphiphiles makes the use of soft templates, such as the air-water interface or fluid-fluid interfaces, possible. In previous investigations, we successfully prepared vesicles from solutions of hexayne glycoside amphiphiles28. Cross-linking of these vesicles was achieved under mild conditions by UV irradiation of the samples. Moreover, we recently prepared self-assembled monolayers from hexaynes with a methyl carboxylate head group and a hydrophobic alkyl tail at the air-water interface in a Langmuir trough. The densely packed molecular precursors were then straightforwardly converted into self-supporting carbon nanosheets at room temperature by UV irradiation. In related approaches defined molecular precursors have recently been used for the preparation of two-dimensionally extended nanosheets at the air-water interface32-38.

The aim of this work is to give a concise, practical overview of the overall synthesis and fabrication steps that allow for the preparation of carbon nanosheets from hexayne amphiphiles. The focus is on the experimental approach and preparative questions.

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Protocol

Caution: Please make sure to consult the relevant materials safety data sheets (MSDS) before the use of any chemical compounds. Some of the chemicals used in these syntheses are acutely toxic and carcinogenic. Prepared nanomaterials may have additional hazards compared to their bulk counterpart. It is imperative to use all appropriate safety practices when performing reactions (fume hood) and personal protective equipment (safety glasses, gloves, lab coat, full length pants, closed-toe shoes). If not otherwise stated the following procedures involve standard Schlenk techniques39.

1. Preparation of the Molecular Precursor29,31

  1. Synthesis of 4-tritylphenyl 10(trimethylsilyl)deca-5,7,9-triynoate (1)
    Note: The required compounds for the subsequently described synthetic steps can be straightforwardly prepared following published literature procedures29,31. Thin layer chromatography (TLC) as well as column chromatography are carried out according to standard laboratory procedures39,40.
    1. Dissolve 1,4-bistrimethylsilylbutadiene (7.82 g, 40.3 mmol) in dry tetrahydrofuran (THF, 50 ml) in an inert atmosphere in a Schlenk tube equipped with a septum. Stir the mixture and cool it to a temperature of 0 °C by immersion of the flask in an ice bath.
    2. Slowly add methyl lithium lithium bromide complex (17.9 ml, 2.2 M in Et2O, 39.3 mmol) to this solution by syringe. Remove the cooling bath and allow the mixture to warm up to room temperature and leave stirring for 30 min.
    3. Immerse the Schlenk tube in an ice bath and cool the solution to a temperature of 0 °C. Slowly add a solution of zinc chloride (57.5 ml, 0.7 M in THF, 40.24 mmol) to the stirred solution. Remove the cooling bath, allow the solution to warm up to room temperature, and stir the mixture for additional 30 min.
    4. In a Schlenk flask, disperse the palladium catalyst PdCl2(dppf) DCM (1.60 g, 1.96 mmol) in dry toluene (300 ml) in an inert atmosphere, and add 4-tritylphenyl 6-bromohex-5-ynoate (10.0 g, 19.63 mmol). Cool this mixture to a temperature of 0 °C by immersion of the Schlenk flask in an ice bath.
    5. Transfer the solution in the Schlenk tube containing the zinc acetylide to the Schlenk flask containing the bromoacetylene derivative and the palladium catalyst by employing a transfer cannula. Leave the mixture stirring and monitor the reaction by TLC39. Consider the reaction complete after TLC (DCM/n-heptane 2:1; coloring with p-anisaldehyde solution39) indicates that the bromoacetylene derivative (Rf: 0.64) is consumed (after approximately 16 hr).
    6. Dilute the reaction mixture with diethylether (Et2O) (300 ml) and transfer it to a separatory funnel. Wash the organic phase three times with saturated ammonium chloride solution (300 ml) and once with saturated sodium chloride solution (300 ml).
    7. Transfer the organic phase from the separatory funnel to an Erlenmeyer flask and dry the organic phase over sodium sulfate (100 g). Remove the sodium sulfate by filtration and concentrate the organic phase in vacuo.
    8. Purify the crude product by column chromatography40 (silica gel; dichloromethane/n-heptane 1:1) in order to obtain the desired product 1 (Rf: 0.33; 7.7 g, 71%) as a light brown solid. Check the purity of (1) by 1H and 13C{1H} NMR spectroscopy29.
  2. Synthesis of 4-tritylphenyl octacosa-5,7,9,11,13,15-hexaynoate (2)
    Note: The synthesis of 4-tritylphenyl octacosa-5,7,9,11,13,15-hexaynoate 2 is carried out over two steps starting from 4-tritylphenyl 10(trimethylsilyl)deca-5,7,9-triynoate due to the observed low stability of the intermediate triyne bromide41.
    1. Dissolve 4-tritylphenyl 10-(trimethylsilyl)deca-5,7,9-triynoate 1 (2.00 g, 3.63 mmol) in dry dichloromethane (DCM, 15 ml) in an inert atmosphere in a 100 ml Schlenk flask equipped with a septum. After dissolution of the compound, add dry acetonitrile (MeCN, 15 ml).
    2. Shield the Schlenk flask from light with aluminum foil and add N-bromosuccinimide (679 mg, 3.81 mmol) as well as silver fluoride (484 mg, 3.81 mmol). Stir the resulting mixture for 8 hr at room temperature and monitor the progress of the reaction by TLC. Consider the reaction complete after TLC (DCM/n-heptane 1:1; detection with p-anisaldehyde solution39 as the coloring agent) indicates that the starting material (1) (Rf: 0.33) is consumed (after approximately 3 hr).
    3. Dilute the reaction mixture with DCM (30 ml) and transfer it into a separatory funnel. Wash the organic phase six times with a 1 M aqueous hydrogen chloride solution (60 ml) and once with a saturated aqueous sodium chloride solution (60 ml).
    4. Transfer the organic phase from the separatory funnel to an Erlenmeyer flask and dry the organic phase over sodium sulfate (50 g). Filter the organic phase and concentrate the filtrate in vacuo to approximately 10 ml, while thoroughly shielding it from light.
    5. Add dry toluene (20 ml) and concentrate the mixture once again in vacuo to a volume of approximately 5 ml.
    6. Dissolve 1-trimethylsilyloctadeca-1,3,5-triyne (2.28 g, 7.26 mmol) in dry THF (20 ml) in an inert atmosphere in a 100 ml Schlenk tube equipped with a rubber septum. Cool the solution to a temperature of 0 °C by immersion of the Schlenk tube in an ice bath.
    7. Slowly add methyl lithium lithium bromide complex (3.22 ml, 2.2 M in Et2O, 7.08 mmol) and keep the temperature of the mixture at 0 °C for 45 min.
    8. To this mixture, slowly add a solution of zinc chloride (10.37 ml, 0.7 M in THF, 7.26 mmol). After the addition is completed, stir the resulting mixture for additional 45 min.
    9. In parallel, disperse the palladium catalyst PdCl2(dppf) DCM (296 mg, 0.36 mmol) in dry toluene (100 ml) in an inert atmosphere in a 500 ml Schlenk flask equipped with a rubber septum. Cool the mixture to a temperature of 0 °C by immersion of the flask in an ice bath.
    10. Slowly add n-butyl lithium (0.29 ml, 2.5 M in n-hexane, 0.73 mmol) to the dispersion of the palladium catalyst in dry toluene. Remove the ice bath after complete addition, stir the mixture for 10 min at room temperature, and thereafter cool it to a temperature of 0 °C.
    11. Add the concentrated toluene solution containing the 4-tritylphenyl 10-bromodeca-5,7,9-triynoate (10 ml, 3.63 mmol) through a syringe simultaneously with the zinc acetylide solution (see above), which is added through a transfer cannula from the Schlenk tube. Shield the flask with the reaction mixture from light with aluminum foil.
    12. Monitor the progress of the reaction by TLC (DCM/n-heptane 1:1; detection with p-anisaldehyde solution39 as the coloring agent). After complete consumption of 4-tritylphenyl 10-bromodeca-5,7,9 triynoate (Rf: 0.34; after approximately 48 hr), dilute the mixture with Et2O (100 ml) and transfer it into a separatory funnel. Wash the organic phase three times with a saturated aqueous ammonium chloride solution (200 ml) and once with a saturated aqueous sodium chloride solution (200 ml).
    13. Transfer the organic phase to an Erlenmeyer flask and dry it over sodium sulfate (80 g). Filter the organic phase and concentrate the filtrate in vacuo.
    14. Purify the crude product by column chromatography40 (silica gel; DCM/n-heptane 1:1) to isolate 4-tritylphenyl octacosa-5,7,9,11,13,15-hexaynoate (2) (Rf: 0.43; 0.60 g, 23%) as a brown solid. Check the purity of (2) by 1H and 13C{1H} NMR spectroscopy29.
  3. Synthesis of Methyl octacosa-5,7,9,11,13,15-hexaynoate (3)
    1. Dissolve 4-tritylphenyl octacosa-5,7,9,11,13,15-hexaynoate (2) (208 mg, 0.289 mmol) in DCM (15 ml) in an inert atmosphere in a 100 ml Schlenk flask, and add methanol (10 ml).
    2. Shield the flask from light with aluminum foil, and add sodium methanolate (31 mg, 0.578 mmol).
    3. Stir the resulting mixture for 30 min at room temperature. Thereafter, dilute with DCM (15 ml), transfer into a separatory funnel, wash once with a 1 M aqueous hydrogen chloride solution (30 ml), and once with saturated aqueous sodium chloride solution (30 ml).
    4. Transfer the organic phase into an Erlenmeyer flask and dry it over sodium sulfate (30 g). Filter the organic phase and concentrate the filtrate in vacuo.
    5. Purify the crude product by column chromatography40 (silica gel; DCM/n-pentane 1:1) to isolate methyl octacosa-5,7,9,11,13,15-hexaynoate (3) (Rf: 0.62) as a yellow solution. Store the product of this reaction as a dilute DCM solution (10 ml) to minimize any decomposition.
    6. Transfer approximately 3 ml of the stock solution to a brown-glass round bottom flask and add CDCl3 (5 ml). Concentrate the solution to approximately 1 ml in vacuo, add CDCl3 (5 ml), and concentrate the solution to approximately 1 ml in vacuo. Perform 1H and 13C{1H} NMR spectroscopy with this solution to check the purity of (3)29.
  4. Freeze-drying of the Methyl Ester and Preparation of a Stock Solution
    1. Prepare a stock solution of defined concentration (c = 1 mM) for experiments at the air-water interface. Transfer a fraction of the dilute solution of methyl octacosa-5,7,9,11,13,15-hexaynoate in DCM to a tared 50 ml brown-glass round bottom flask and concentrate the solution to approximately 1 ml in vacuo.
    2. Connect the flask to a vacuum line and immerse the flask in liquid nitrogen in order to freeze the concentrated solution. After the mixture has completely solidified, apply vacuum (approximately 5 x 10-3 mbar) to the flask and remove the flask from the liquid nitrogen.
    3. After complete removal of the residual solvent, weigh the flask while shielding it from light. Prepare a stock solution of defined concentration c = 1 mM by addition of the appropriate amount of chloroform. Shield the stock solution from light and store it at a temperature of -15 °C.

2. Film Formation of the Hexayne Amphiphile at the Air-water Interface

Note: All experiments at the air-water interface are carried out with computer-interfaced polytetrafluoroethylene Langmuir troughs with one (or two) barriers. The troughs are equipped with a surface pressure microbalance with a filter paper Wilhelmy plate. An external thermostat is used to maintain the temperature of the ultrapure water subphase at 20 °C. The Langmuir troughs are placed in a sealed box to avoid the contamination of the interface. Before measurements are carried out, the purity of the bare surface is checked by compression. All used experimental Langmuir setups are calibrated prior to use by employing a two-point calibration method: the bare water surface for 0 mN/m as well as the second-order transition from a tilted to a non-tilted state of stearic acid42, that is not sensitive to temperature changes in the used temperature range. This ensures reliable and comparable measurements.

  1. Measurement of the Surface Pressure-area Isotherm and Determination of the Compressibility Modulus
    1. Use a syringe to carefully spread 100 µl of the dilute stock solution of (3) in DCM/chloroform (c = 1 mmol/L) on the surface of the ultrapure water in a Langmuir trough.
    2. Leave the Langmuir trough to equilibrate for 15 min allowing the solvent to evaporate.
    3. Compress the layer with a constant compression rate of 5 Å/(molecule x min) by reducing the surface area of the Langmuir trough with the barriers. Simultaneously measure the surface pressure with the surface pressure microbalance equipped with a filter paper Wilhelmy plate. Continue the compression of the layer until a drop in the surface pressure indicates the collapse of the film.
    4. Repeat the surface pressure-area isotherm measurements as described in 2.1.1-2.1.3 two times with a clean Langmuir trough to ensure the reproducibility of the results.
    5. Determine the compressibility modulus from the isotherm of the layer according to the following relation43
      Cs-1 = -A(∂∏/∂A)T
      where A is the mean molecular area and ∏ the surface pressure.
  2. Investigation of the Stability of the Layer Below and Above the Plateau in the Isotherm
    1. Spread an aliquot of 100 µl of the dilute stock solution of (3) on ultrapure water in a Langmuir trough.
    2. Leave the Langmuir trough to equilibrate for 15 min allowing the solvent to evaporate.
    3. Compress the layer to a surface pressure of 8 mN/m with a constant compression rate of 5 Å/(molecule x min) by reducing the surface area of the Langmuir trough with the barriers. Continue the compression of the layer until the measured surface pressure reaches 8 mN/m and set the barriers so that this surface pressure is kept at a constant value.
    4. Monitor the development of the surface area as the barriers of the Langmuir trough adjust over the course of 45 min to maintain a constant surface pressure of 8 mN/m.
    5. Compress the layer to a surface pressure of 23 mN/m with a constant compression rate of 5 Å/(molecule x min) by further reducing the surface area of the Langmuir trough with the barriers. Set the barriers so that the surface pressure is kept constant at 23 mN/m.
    6. Monitor the development of the surface area as the barriers of the Langmuir trough adjust over the course of 45 min to maintain a constant surface pressure of 23 mN/m.
  3. Characterization of the Layer by Infrared Reflection-absorption Spectroscopy
    Note: In order to record infrared (IR) spectra, an FT-IR spectrometer equipped with a liquid nitrogen-cooled mercury cadmium telluride (MCT) detector is employed and the latter is attached to an external air/water reflection unit. The IR beam is guided out of the spectrometer and focused onto the ultrapure water surface of the thermostated Langmuir trough. Light of p-polarization at an angle of incidence below (40°) the Brewster angle was used for the measurements. The Langmuir trough for IR measurements consists of two compartments: in one compartment, the sample monolayer system is spread, while the other compartment contains the ultrapure water subphase as a reference. A computer-controlled shuttle system is used to move the trough, so that either the sample or the reference compartment is illuminated. To eliminate the water vapor signal from the spectra, calculate the reflection-absorption spectrum according to log(R/R0), with the single-beam reflectance spectrum from the reference compartment (R0) as a background for the single-beam reflectance spectrum of the monolayer (R) from the sample compartment. Maintain the water vapor pressure constant by placing the whole experimental setup into a hermetically sealed box. In all experiments, use a resolution and scanner speed of 8 cm-1 and 20 kHz, respectively. Use a wire grid polarizer to polarize the incident IR beam. Co-add the spectra with p-polarized light over 400 scans. Perform a baseline-correction to allow for a comparison of the spectra.
    1. Spread an aliquot of 100 µl of the dilute stock solution of (3) on ultrapure water in a Langmuir trough comprising an infrared reflection-absorption (IRRA) spectroscopy setup.
    2. Leave the Langmuir trough to equilibrate for 15 min allowing the solvent to evaporate.
    3. Compress the layer to a surface pressure of 1 mN/m with a constant compression rate of 5 Å/(molecule x min) by reducing the surface area of the Langmuir trough with the barriers. Monitor the surface pressure by means of the surface pressure microbalance with a filter paper Wilhelmy plate and set the barriers of the Langmuir trough so that a surface pressure of 1 mN/m is maintained.
    4. Record an IR spectrum with p-polarized light at an angle of incidence of 40°44,45.
    5. Compress the layer at a constant compression rate of 5 Å/(molecule x min) by further reducing the surface area of the Langmuir trough with the barriers so that surface pressures of 3, then 5, and finally 8 mN/m are obtained as indicated by the surface pressure microbalance. Record spectra with p-polarized light at an angle of incidence of 40° for each of these surface pressures while keeping the latter constant44,45.

3. Carbonization of a Hexayne Amphiphile Monolayer at Room Temperature

Note: All experiments at the air-water interface are performed as described above. UV irradiation is carried out using a 250 W gallium-doped metal halide UV lamp (UV-Light Technology, Birmingham, United Kingdom). For carbonizations of films at the air-water interface, the box enclosing the Langmuir trough is carefully removed or opened at the side, and the lamp is placed 50 cm away from the water surface while ensuring that the air-water interface is covered in the cone of UV light. During illumination, the temperature of the subphase is maintained at 20 °C using the thermostat of the Langmuir trough. All carbonization experiments are performed at a surface pressure of 8 mN/m. At this pressure a tight packing of the amphiphiles is ensured while, at the same time, the monolayer displays a high stability.

  1. Investigation of the Carbonization by IRRA Spectroscopy and Isotherm Measurement
    Note: IRRA spectra are recorded as described above44,45.
    1. Spread an aliquot of 100 µl of the dilute stock solution of (3) on ultrapure water in the Langmuir trough comprising an infrared reflection-absorption spectroscopy (IRRA) setup.
    2. Leave the Langmuir trough to equilibrate for 15 min allowing the solvent to evaporate.
    3. Compress the layer at a constant compression rate of 5 Å/(molecule x min) by reducing the surface area of the Langmuir trough with the barriers so that a surface pressure of 8 mN/m is obtained as measured by the surface pressure microbalance equipped with a filter paper Wilhelmy plate. Set the barriers of the Langmuir trough so that a surface pressure of 8 mN/m is maintained.
    4. Record an IR spectrum with p-polarized light at an angle of incidence of 40°44,45.
    5. Carefully remove the box enclosing the Langmuir trough. Mount the UV lamp to a support stand and place it approximately 50 cm away from the water surface while ensuring that the interface is covered in the cone of the UV light.
    6. After confirming that the monolayer is still compressed at a surface pressure of 8 mN/m as measured by the surface pressure microbalance, set the barriers of the Langmuir trough so that they are fixed at the current position.
    7. Expose the air-water interface to UV light. Stop the irradiation by turning the lamp off after a total of 40 min of irradiation. Monitor and record the change of the surface pressure by the surface pressure microbalance equipped with a filter paper Wilhelmy plate throughout the course of the irradiation.
    8. Enclose the Langmuir trough in the sealed box in order to avoid a contamination of the interface. Leave the setup to equilibrate for 30 min. Set the barriers of the Langmuir trough to maintain the surface pressure observed by the surface pressure microbalance after irradiation.
    9. Record an IR spectrum with p-polarized light at an angle of incidence of 40°44,45.
  2. Investigation of the Carbonized Film by Brewster Angle Microscopy
    Note: Brewster angle microscopy (BAM) experiments are performed on a custom-made computer-interfaced Langmuir trough coupled with a Brewster angle microscope as described elsewhere46,47. The surface pressure isotherms are continuously measured throughout the course of the microscopy experiments. The lateral resolution is approximately 4 µm with the employed setup. Simple image processing software is used to improve the contrast of the micrographs.
    1. Spread an aliquot of 100 µl of the dilute stock solution of (3) on ultrapure water in the Langmuir trough aligned with the Brewster angle microscope.
    2. Leave the Langmuir trough to equilibrate for 15 min, allowing the solvent to evaporate.
    3. Compress the layer to a surface pressure of 8 mN/m with a constant compression rate of 5 Å/(molecule x min) by reducing the surface area of the Langmuir trough with the barriers. Monitor the surface pressure by means of the surface pressure microbalance equipped with a filter paper Wilhelmy plate and set the barriers of the Langmuir trough so that a surface pressure of 8 mN/m is maintained.
    4. Record BAM micrographs at this surface pressure46,47.
    5. Carefully open the box enclosing the Langmuir trough. Mount the UV lamp to a support stand and place it approximately 50 cm away from the water surface while ensuring that the interface is covered in the cone of the UV light.
    6. Make sure that the monolayer is still compressed at a surface pressure of 8 mN/m and fix the position of the barriers of the Langmuir trough.
    7. Expose the air-water interface to UV light. Stop the irradiation by turning the lamp off after a total of 40 min. Monitor and record the change of the surface pressure throughout the course of the irradiation.
    8. Record BAM micrographs after irradiation46,47.
    9. Use a needle to manipulate the carbon nanosheet at the air-water interface, and use the BAM to monitor the movement of the carbon nanosheet. Applying pressure to the carbon nanosheet leads to a rupture of the sheet.
    10. Record BAM micrographs of the ruptured sheet46,47.
  3. Langmuir-Blodgett Transfer of an Uncarbonized Layer and a Carbon Nanosheet from the Air-water Interface to a Solid Substrate
    Note: The sapphire (Al2O3) substrates are cleaned prior to transfer by a basic piranha treatment and stored in ultrapure water prior to use48. A pair of tweezers attached to a mechanical arm is placed vertically above a Langmuir trough with an appropriately deep cavity for the Langmuir-Blodgett (LB) transfer of layers from the air-water interface49. If the transfer of a non-carbonized as well as a carbonized sample is intended within the same experiment, as described here, two pairs of tweezers need to be attached to the mechanical arm at different heights so that one substrate can be removed while the second substrate remains immersed in the subphase. The Langmuir-Schäfer (LS) transfer was carried out manually with holey carbon transmission electron microscopy (TEM) grids as a support50.
    1. Install two sapphire substrates with the two pairs of tweezers, immerse the substrates in the subphase, and thoroughly clean the air-water interface before spreading.
    2. Carefully spread an aliquot of 100 µl of the dilute stock solution of (3) in DCM/chloroform on ultrapure water in the Langmuir trough.
    3. Leave the Langmuir trough to equilibrate for 15 min allowing the solvent to evaporate.
    4. Compress the layer to a surface pressure of 8 mN/m with a constant compression rate of 5 Å/(molecule x min) by reducing the surface area of the Langmuir trough with the barriers. Monitor the surface pressure by means of the surface pressure microbalance equipped with a filter paper Wilhelmy plate and set the barriers of the Langmuir trough so that a surface pressure of 8 mN/m is maintained.
    5. In order to transfer the non-carbonized monolayer to a sapphire substrate, maintain the monolayer at a surface pressure of 8 mN/m as indicated by the surface pressure microbalance and pull the mechanical arm up at a speed of 1.2 mm/min until the first substrate is completely removed from the subphase. The second substrate needs to remain immersed in the subphase.
    6. Carefully retrieve the first substrate carrying the non-carbonized layer from the pair of tweezers, store it under protection from light and employ it for the intended application when needed.
    7. Carefully open the box enclosing the Langmuir trough. Mount the UV lamp to a support stand and place it approximately 50 cm away from the water surface, while ensuring that the interface is covered in the cone of the UV light.
    8. Ensure that the monolayer is still compressed at a surface pressure of 8 mN/m and fix the position of the barriers.
    9. Expose the air-water interface to UV light. Stop the irradiation by turning the lamp off after a total of 40 min of irradiation. Monitor and record the change of the surface pressure as measured by the surface pressure microbalance throughout the course of the irradiation.
    10. In order to transfer the carbonized film to a sapphire substrate, set the barriers of the Langmuir trough so that the surface pressure measured after irradiation is maintained. While keeping the surface pressure constant by the barriers of the Langmuir trough, retract the mechanical arm holding the substrate from the interface at a speed of 1.2 mm/min until the substrate is completely removed from the subphase.
    11. Carefully retrieve the substrate carrying the carbonized layer from the pair of tweezers.
    12. Hold a holey carbon TEM grid with a pair of tweezers and perform a manual LS transfer of the carbonized layer from the air-water interface50. Let the sample slowly dry in a gentle stream of nitrogen and perform scanning electron microscopy (SEM) imaging.
    13. Record UV/Vis absorption spectra of the non-carbonized layer and the carbon nanosheet on a UV/Vis spectrometer at a scan speed of 400 nm per minute. Use an empty sapphire substrate as the reference.

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Results

The 13C nuclear magnetic resonance (NMR) spectrum of the prepared precursor molecule 3 displays the 12 sp-hybridized carbon atoms of the hexayne segment with the corresponding chemical shifts of δ = 82-60 ppm (Figure 1b). Moreover, the signals at δ = 173 ppm and at δ = 52 ppm are assigned to the carbonyl and methyl carbon of the ester, respectively. The signals between δ = 33-14 ppm are ascribed to the aliphatic carbons of the dodecyl ...

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Discussion

The desired hexayne amphiphile (3) is straightforwardly prepared by the sequential bromination52,53 and Pd-catalyzed elongation30,31 of the alkyne segment, followed by a final deprotection reaction of the tritylphenyl ester (2) (Figure 1a)29. The successful synthesis is confirmed by the 13C NMR spectrum (Figure 1b) as well as the UV-Vis absorption spectrum (Figure 1c)31,54. This demonstrates the facile...

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Disclosures

The authors have nothing to disclose.

Acknowledgements

Funding from the European Research Council (ERC Grant 239831) and a Humboldt Fellowship (BS) is gratefully acknowledged.

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Materials

List of materials used in this article
NameCompanyCatalog NumberComments
Methyllithium lithium bromide complex (2.2 M solution in diethylether)Acros18129-1000air-sensitive, flammable
Zinc chloride (0.7 M solution in THF)Acros38945-1000air-sensitive, flammable
1,1'-Bis(diphenylphosphino)ferrocene]
dichloropalladium(II), DCM adduct 		Boron MolecularBM187
N-BromosuccinimideAcros10745light-sensitive
Silver fluorideFluorochem002862-10glight-sensitive
n-Butyllithium (2.5 M solution in hexanes)Acros21335-1000air-sensitive, flammable
Sodium methanolateAcros17312-0050
Tetrahydrofuran (unstabilized, for HPLC)Fisher ChemicalsT/0706/PB17This solvent was dried as well as degassed using a solvent purification system (Innovative Technology, Inc, Amesbury, MA, USA)
Toluene (for HPLC)Fisher ChemicalsT/2306/17This solvent was dried as well as degassed using a solvent purification system (Innovative Technology, Inc, Amesbury, MA, USA)
Acetonitrile (for HPLC)Fisher ChemicalsA/0627/17This solvent was dried as well as degassed using a solvent purification system (Innovative Technology, Inc, Amesbury, MA, USA)
Dichloromethane (Extra Dry over Molecular Sieve)Acros34846-0010
Chloroforme (p.a.)VWR International1.02445.1000
PentaneReactolab99050Purchased as reagent grade and distilled once prior to use
HeptaneReactolab99733Purchased as reagent grade and distilled once prior to use
DichloromethaneReactolab99375Purchased as reagent grade and distilled once prior to use
DiethyletherReactolab99362Purchased as reagent grade and distilled once prior to use
Geduran silica gel (Si 60, 40-60 µm)Merck1115671000
Langmuir troughR&K, Potsdam
Thermostat E1 Medingen
Hamilton syringe Model 1810 RN SYR
Vertex 70 FT-IR spectrometer Bruker
External air/water reflection unit (XA-511) Bruker
UV lamp (250 W, Ga-doped metal halide bulb)UV-Light Technology
Brewster angle microscope (BAM1+) NFT Göttingen
Sapphire substratesStecher Ceramics
Quantifoil holey carbon TEM gridsElectron Microscopy Sciences
Nuclear magnetic resonance spectrometer (Bruker Avance III 400)Bruker
JASCO V-670 UV/Vis spectrometerJASCO
Scanning Electron Microscope (Zeiss Merlin FE-SEM)Zeiss

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