Glaucoma is one of the major causes of blindness in the world. Elevated intraocular pressure is a major risk factor. Laser photocoagulation induced ocular hypertension is one of the well established animal models. This video demonstrates how to induce ocular hypertension by Argon laser photocoagulation in rat.
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Method Article
Laser-Induced Chronic Ocular Hypertension Model on SD Rats
In This Article
Summary
Abstract
Glaucoma is one of the major causes of blindness in the world. Elevated intraocular pressure is a major risk factor. Laser photocoagulation induced ocular hypertension is one of the well established animal models. This video demonstrates how to induce ocular hypertension by Argon laser photocoagulation in rat.
Protocol
Check and prepare the equipments
- Anaesthetize the rat by intra-peritoneal injection of ketamine (80mg/kg) and xylazine (8mg/kg) (volume ratio at 2:1).
- Apply one drop of 0.5% alcaine to the rat eyes as topical anesthetics before laser photocoagulation.
- Position the rat and expose the target veins with a curved forceps. Use the footstep to start laser photocoagulation. Apply bout 60 laser spots around the limbal vein (except the nasal area) and 15-20 laser spots on each episcleral aqueous humor drainage vein.
Laser photocoagulation
- Anaesthetize the rat by intra-peritoneal injection of ketamine (8....
Access restricted. Please log in or start a trial to view this content.
Discussion
After two laser treatments with 7 days apart, we can elevate the IOP of laser photocoagulated eye by 50% compare with control eye. The elevated IOP can sustain for at least for 3 month after first laser treatment. This rat model of elevated intraocular pressure provide valuable opportunities to study the mechanisms of pressure-induced retinal ganglion cell loss and optic nerve damage that mimic the pathological change in human glaucoma.
....Access restricted. Please log in or start a trial to view this content.
Disclosures
The authors have nothing to disclose.
Materials
| Name | Company | Catalog Number | Comments | |
|---|---|---|---|---|
| ULTIMA® 2000SE Argon Laser | Tool | Coherent Inc. | ||
| Protective goggles | Other | Coherent Inc. |
References
- Chan, H. -C., Chang, R. C. C., Koon-Ching Ip, A., Chiu, K., Yuen, W. -H., Zee, S. -Y., So, K. -F. Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma. Experimental Neurology. 203, 269-273 (2007).
- Ji, J. -Z., Elyaman, W., Yip, H. K., Lee, V. W. H., Yick, L. -W., Hugon, J., So, K. -F.
Access restricted. Please log in or start a trial to view this content.
Reprints and Permissions
