A Minimally Invasive, Fast Spinal Cord Lateral Hemisection Technique for Modeling Open Spinal Cord Injuries in Rats

Summary

Here, we describe a new, fast technique modeling open spinal cord injury in rats that eliminates laminectomy. Lateral hemisection is performed while viewing through a microscope. The technique is versatile and can also be used in the cervical, thoracic, and lumbar regions of the spinal cord of other animals.

Abstract

Open spinal cord injury techniques modeling laceration-like injuries are time-consuming and invasive because they involve laminectomy. This new technique eliminates laminectomy by removing two spinous processes and lifting, then tilting the caudal vertebral arch. The surgical area opens up without the need for laminectomy. Lateral hemisection is then performed with direct visible control under a microscope. The trauma is minimized, requiring only a small bone wound.

This technique has several advantages: it is faster and, therefore, less of a burden for the animal, and the bone wound is smaller. Because the laminectomy is eliminated, there is less chance for unwanted injury to the spinal cord, and there are no bone splinters that can cause problems (bone splinters embedded in the spinal cord can cause swelling and secondary damage). The vertebral canal remains intact. The main limitation is that the hemisection can only be performed in the intervertebral spaces.

The results show that this technique can be performed much faster than the traditional surgical approach, using laminectomy (11 min vs. 35 min). This technique can be useful for researchers working with animal models of open spinal cord injury as it is widely adaptable and does not require any additional specialized instrumentation.

Introduction

Spinal cord injuries (SCIs) are unfortunately prevalent injuries in humans. SCIs can be complicated in different ways, for example, by infections, and it is clinically important to study these injuries¹. Because there is no single, definite cure for SCIs, animal models are still needed to further the understanding of researchers and advance possible treatments^2,3. Although closed injuries are most commonly modeled (compression and contusion), it is clinically important to understand lacerations, which can only be modeled in open injuries⁴. Open-wound models using transection or hemisection can be used to demonstrate a more precise localization of a wound compared to closed injury models, owing to the nature of the injury (contusion vs. surgical cut). Open-wound experiments can shed light on more specific neuronal injuries in a controlled, reliable, and replicable way⁵. The complete or partial transection of the spinal cord is a widely used open-wound technique and can be viewed in detail in the article by Brown and Martinez⁶.

When studying open spinal cord injury in rats, several animals presented problems that arose from the surgery: bone splinters from the laminectomy became embedded in the spinal cord and caused swelling; the larger bone wound needed a long time to heal; the surgery took too long. An alternate surgical technique was developed to eliminate these problems. The goal was to develop a faster technique that is gentler for the animal. This newly developed technique is much faster than traditional SCI techniques. The surgical approach is minimally invasive, resulting in a smaller bone wound while eliminating problems arising from the laminectomy.

All open-wound techniques involve opening the dura⁷. Several recent studies have examined different, newly developed techniques, aiming to improve the previous methods^8,9. Even though the opening of the dura cannot be excluded using this new technique, it causes a smaller wound on the dura while offering a reliable, controlled injury of the spinal cord. Consulting the literature on spinal cord injury techniques, many authors tried to minimize the time of surgery by implementing minor changes to the original technique¹⁰. Laminectomy is always part of these surgical procedures, although it is time-consuming and requires a larger bone wound to be made⁶. This surgical technique can be appropriate for researchers using open wound spinal cord injury models, specifically complete transection or lateral hemisection performed in the intervertebral spaces (Figure 1).

Protocol

All animal procedures were carried out according to the EU Directive (2010/63/EU) and were approved by the animal ethics committee of the Hungarian National Food Chain Safety Office (PEI/001/2894-11/2014). All applicable institutional and governmental regulations concerning the ethical use of animals were followed during this study.

1. Preparation before surgery

1. Sterilize all instruments used during the procedure (see the Table of Materials) and disinfect the surfaces where the work is to be performed prior to the procedure.
2. Inject a single dose of subcutaneous antibiotics prophylactically.
   NOTE: See the Table of Materials for antibiotic dosage details.
3. Leave the animals in the operating room for 1 h to acclimate them and decrease their stress prior to surgery.
4. Anesthetize the rat via an intramuscular injection of a combination of ketamine and xylazine (ketamine 80 mg/kg body weight (bw) and xylazine 8 mg/kg bw).
   NOTE: In addition to anesthesia, the ketamine-xylazine combination provides sufficient analgesia for this procedure. The analgesia regimen can be modified per the institutional animal use guidelines.
5. Keep the rat warm during the procedure using a heated table or infrared light and keep the eyes moist throughout the anesthesia using ophthalmic ointment (reapply as necessary).
6. Fixate the animal on the operating table using surgical tape on its front and back paws and tail, and depending on the site of injury, on the neck as well. If necessary, place the rat in a stereotaxic frame to stabilize it during surgery.
7. Using sterile surgical suture, place a loop around the upper front teeth of the rat and fixate this on the edge of the operating table.
8. Pull out the tongue sideways for airway management.
9. Shave the fur on the back, at least 2 cm in each direction of where the incision will be made.
10. Disinfect the skin of the surgical area at least three times, using a povidone-iodine solution and sterile gauze. Take special care to soak the fur surrounding the area. Secure the surgical site with a sterile drape. 
11. Assess the adequacy of the anesthesia before placing the first incision by pinching the toes and the tail of the animal. Continue monitoring the adequacy of anesthesia during the entire procedure.

2. Surgery

1. Place the skin incision using a scalpel blade 20. To open the surgical area, place a 2-2.5 cm long incision along the spine, cutting through all the layers of the skin. Position this incision parallel to the spinal column using the L1 vertebra as a midpoint, making it extend ~1 cm in both the cranial and caudal direction along the spinal column.
2. Mobilize the sides of the wound by cutting through the connective tissue surrounding the muscles.
3. Place two parallel incisions along the spine, penetrating the periosteum. Place the incisions right next to the spinal processes on both sides, spanning the distance between the Th13 and L1 vertebrae.
4. Dissect the muscles attached to the vertebrae with the aid of a raspatorium until all the spinal ligaments are visible. Put in place a retractor.
5. Remove the spinal processes of the 13^th thoracic vertebra and the 1^st lumbar vertebra using dental bone forceps to visualize the entire surgical area. Hereon, control the procedure by viewing an enlarged (4x-16x magnification) microscopic image.
6. Use sterile gauze to control bleeding throughout the procedure, when necessary.
7. Carefully lift the remainder of the L1 spinal processes, elevating the L1 vertebral arch. Sever the ligamentum flavum to access the spinal cord. Raise the caudal spinal processes further, allowing access to the spinal dura mater, which is also severed. Tip the caudal spinal processes in the cranial direction to visualize the pia mater.
8. Look through the pia mater for the posterior median vein, showcasing the midline of the spine.
9. Using the vein as a directional bisector, place an incision using a microsurgical scalpel while sparing the vein. Place the incision under the vein, in the transversal plane through the anteroposterior diameter of the spinal cord. Sever half of the spinal cord by moving the blade laterally away from the centerline.
   NOTE: The incision is unilateral, on the right side, at the 4^th lumbar segment.
10. Try to place the incision to avoid cutting the anterior spinal artery. Ensure excess pressure is not applied on the vertebral body when cutting the spinal cord to spare the anterior spinal artery on the ventral side of the spinal cord.

Figure 1: Artwork showing the steps of the new open SCI technique in rats. (A) The exposed vertebrae. (B) Spinal processes removed (Th13 and L1). (C) The lifted and tilted vertebral arch of the L1 vertebra. (D) Hemisection performed on the right side, with hemisected spinal cord shown separately, zoomed in. Please click here to view a larger version of this figure.

11. Do not close the dura mater directly during wound closure. Tightly suture (suture size 4-0) the muscles along the spinal processes, indirectly closing the small wound on the dura mater.
12. Close the dorsal connective tissue layer with sutures.
13. Finally, suture the skin around the incision site.

3. Postsurgical care and follow-up

1. Allow the animals to awaken in their cages. Keep the animal(s) warm using a heat lamp in addition to the temperature-controlled room. Do not leave the rats alone after they awaken after surgery, and do not put them together with other rats in the same cage.
2. Monitor their respiratory rate at least every 10 min until they are fully awake. If necessary, apply gentle stimulation (e.g., rub the head) to aid their awakening from anesthesia.
3. When the animals are alert and appropriately active, transport them safely back to the animal house.
4. Keep the rats under close surveillance for the first 24 h post surgery. Following the first 24 h post surgery, check the animals at least twice a day until the end of the experiment, monitoring for signs of distress.
5. Thoroughly assess them once a day for signs of distress using the relevant institutional animal welfare protocol and take special care to check their wounds for signs of infection and inflammation.
   NOTE: Stress and infection affect the welfare of the animals and the outcome of experiments.
6. Administer antibiotics subcutaneously every day until the end of the experiments. Keep the animals in sterile cages, one animal per cage, and give food and water ad libitum, same as before. At the end of the experiments (or if any serious adverse reaction is observed during the timeframe of the experiment), euthanize the animals humanely, in accordance with the relevant institutional animal welfare protocol.
   NOTE: Here, the animals were euthanized (in a deep sleep induced by the combination of ketamine-xylazine) by first administering a physiological saline perfusion (1/3 mL/g bw) followed by a 4% paraformaldehyde perfusion (1 mL/g bw).

Results

Following the hemisection, the rats show paralysis in the ipsilateral hindlimb (in vivo proof of successful hemisection). Thorough specimen evaluation can only be done following the removal of the spinal cord (see Figure 2, where the removed spinal cord can be seen from both the ventral and dorsal sides).

Discussion

This minimally invasive spinal cord injury technique was developed when studying spinal cord-injured rats, and the team was faced with problems arising from the surgery itself (bone splinters from the laminectomy causing compression and damaging the spinal cord, surgery taking too long, slow healing of a large bone wound). By eliminating the laminectomy, the procedure became much faster (11 min vs. 35 min), the structure of the vertebral canal remained intact, the bone wound was much smaller, and there were no bone splin...

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Augmentin (1,000 mg/200 mg powder)	GlaxoSmithKline, UK		One-time dose of s.c. antibiotics prophylactically (10 mg of amoxicillin and 2 mg clavulanic acid; Augmentin 1,000 mg/200 mg powder). Every day following surgery, 10 mg of amoxicillin and 2 mg of clavulanic acid (Augmentin 1,000 mg/200 mg powder) per day per animal
Betadine	EGIS, Hungary		Disinfect the skin of the surgical area using a povidone-iodine solution
Calypsol (50 mg/mL)	Richter Gedeon, Hungary		Anesthesia: combination of ketamine 80 mg/kg and xylazine 8 mg/kg intramuscularly
CP XYLAZIN 2% (20 mg/mL)	Produlab Pharma B.V., the Netherlands		Anesthesia: combination of ketamine 80 mg/kg and xylazine 8 mg/kg intramuscularly
Dental bone forceps	Dentech, Hungary	BS 0127	Remove the spinous processes of the 13th thoracic vertebra and the 1st lumbar vertebra using dental bone forceps
dental surgical micromotor	W&H, Austria	MF-TECTORQUE	Using a dental surgical micromotor, a laminectomy is performed at the L1 vertebra
optical microscope	Zeiss, Germany	OPMI19-FC	Control the procedure by viewing an enlarged (16x magnification) microscopic image
physiological saline solution (0.9% NaCl)	Fresenius Kabi, Germany		Keep the rat's eyes moist throughout the entire anesthesia using physiological saline solution drops (reapply as necessary)
raspatorium	Dentech, Hungary	FK 1164	Dissect the muscles attached to the vertebrae with the aid of a raspatorium, until all the spinal ligaments are visible.
retractor	Dentech, Hungary	RT 1253
scalpel	Dentech, Hungary	BB 173
scalpel	Dentech, Hungary	BB 184
scalpel blade 12	B. Braun, Germany	12
scalpel blade 20	B. Braun, Germany	20
sterile cut gauze 10 x 10 cm	Sterilux, Hartmann, Germany
sutures (monofilament, synthetic; absorbable and nonabsorbable), size: 4-0	B. Braun, Germany
tweezer (13 cm)	Dentech, Hungary	BD 1555
tweezer (delicate tissue forceps)	Dentech, Hungary	BD 1670