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JoVE Journal Neuroscience
A Minimally Invasive, Fast Spinal Cord Lateral Hemisection Technique for Modeling Open Spinal Cord Injuries in Rats
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A Minimally Invasive, Fast Spinal Cord Lateral Hemisection Technique for Modeling Open Spinal Cord Injuries in Rats

Article DOI: 10.3791/63534-v • 04:50 min • March 23rd, 2022
March 23rd, 2022 •
1, 2,3, 4, 5, 2, 6
1Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University, 2Department of Oral Biology, Faculty of Dentistry, Semmelweis University, 3Centre for Translational Medicine, Semmelweis University, 4Department of Molecular Biology, Faculty of Medicine, Semmelweis University, 5Institute of Translational Medicine, Faculty of Medicine, Semmelweis University, 6Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University

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Summary
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Here, we describe a new, fast technique modeling open spinal cord injury in rats that eliminates laminectomy. Lateral hemisection is performed while viewing through a microscope. The technique is versatile and can also be used in the cervical, thoracic, and lumbar regions of the spinal cord of other animals.

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Minimally Invasive Fast Spinal Cord Lateral Hemisection Technique Modeling Open Spinal Cord Injuries In Rats Laminectomy Spinous Processes Caudal Vertebral Arch Surgical Area Opening Visible Control Microscope Trauma Minimization Small Bone Wound Advantages Faster Procedure Reduced Burden For Animals Smaller Bone Wound Elimination Of Laminectomy Risks Intact Vertebral Canal Limitation Of Intervertebral Spaces Shorter Surgical Approach Time (11 Min Vs. 35 Min) Usefulness For Researchers Working With Animal Models Of Open Spinal Cord Injury
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