Preparation of Platelet-Rich Plasma from Human Peripheral Blood

Abstract

Source:
Sathish Muthu<sup>1,2,3

1</sup>Orthopaedic Research Group, Coimbatore.

²Department of Biotechnology, Faculty of Engineering, Karpagam Academy of Higher Education.

³Department of Orthopaedics, Government Medical College.

This video demonstrates the preparation of high-quality Platelet-Rich Plasma (PRP) by screening for platelet count, collecting and centrifuging blood, and isolating platelets. This PRP is used in therapeutic applications for tissue regeneration and healing.

Protocol

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board.

1. Sample collection

1. Screen the patients using preliminary blood counts to rule out thrombocytopenia and establish baseline cell counts. To do this, use a clinical-grade hemocytometer for cell counting. Ensure the platelet counts are above 150,000 cells/µL.

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