Preparation of Platelet-Rich Plasma from Human Peripheral Blood

Begin with human peripheral blood in a conical tube.

Centrifuge the tube at a low speed to separate the blood into three layers: red blood cells at the bottom, the buffy coat in the middle, and platelet-containing plasma at the top.

Transfer the plasma layer to a fresh tube and centrifuge it at a high speed to pellet the platelets.

Transfer the plasma supernatant to a new tube.

Collect the platelet pellet and reconstitute it with an appropriate volume of the plasma supernatant to obtain platelet-rich plasma or PRP, which contains a high concentration of platelets.

To begin, place the centrifugation tubes containing the anticoagulated blood in a clinical grade tabletop centrifugation machine set to room temperature within a sterile environment. Run the initial soft spin at 100 g for 15 minutes to separate the anticoagulated blood into three layers: red blood cells at the bottom, plasma on top, and the buffy coat in the middle.

Using an 18-gauge sterile spinal needle, pipette the top layer of plasma from the trilayer sample in a sterile manner. Collect the plasma into separate conical bottom centrifugation tubes, ensuring each tube holds 10 milliliters of plasma.

Then, place the 15-millil