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Biology

Counting and Determining the Viability of Cultured Cells

doi: 10.3791/752 Published: June 23, 2008

Summary

Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.

Abstract

Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A hemacytometer is a thick glass slide with a central area designed as a counting chamber. Cell suspension is applied to a defined area and counted so cell density can be calculated.

Protocol

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The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.

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Disclosures

The authors have nothing to disclose.

Counting and Determining the Viability of Cultured Cells
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Cite this Article

Ricardo, R., Phelan, K. Counting and Determining the Viability of Cultured Cells. J. Vis. Exp. (16), e752, doi:10.3791/752 (2008).More

Ricardo, R., Phelan, K. Counting and Determining the Viability of Cultured Cells. J. Vis. Exp. (16), e752, doi:10.3791/752 (2008).

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