The complete text protocol for this experimental approach is available in <a href="http://www.mrw.interscience.wiley.com/emrw/9780471143031/cp/cpcb/article/cb1110/current/abstract ]">Current Protocols in Cell Biology</a>.

The authors have nothing to disclose.

preparation and fractionation of xenopus laevis egg extracts

Preparation and Fractionation of Xenopus laevis Egg Extracts

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical ...

In a previous video, we demonstrated how to obtain and de jelly eggs from mature XUS labis. Females crude extracts from these eggs provide a useful source of cytoplasmic and organelle material for the in vitro reconstitution of cellular events such as nuclear assembly, nuclear protein import, and DNA replication. Hi, my name's Marie Cross and I'm a graduate student in Dr.Maureen Power's lab in the Department of Cell Biology at Emory University in Atlanta, Georgia.Today we will show you how to fractionate egg extract into soluble cytoplasmic and light membrane fractions. This procedure involves the following steps, lysing the eggs, collecting the crude extracts, fractionating crude egg extracts, and collecting cytoplasmic extract and collecting the light membrane fraction of the egg extract. So let's get started.Let's begin by lysing eggs and collecting crude extracts before egg lysis. A transfer pipette must be made by breaking a pasture pipette and then carefully inserting the broken end into a pipette bulb. Transfer the eggs into a 15 mil polypropylene tube.A wide mouth pipette is required to avoid lysing the eggs. Next, centrifuge the eggs for 30 seconds. In a clinical centrifug

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Emory University.  Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

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Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

Preparation and Fractionation of Xenopus laevis Egg Extracts

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