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Biology

Preparation and Fractionation of Xenopus laevis Egg Extracts

doi: 10.3791/891 Published: August 27, 2008

Summary

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

Abstract

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

Protocol

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The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.

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Disclosures

The authors have nothing to disclose.

Preparation and Fractionation of Xenopus laevis Egg Extracts
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Cite this Article

Cross, M. K., Powers, M. Preparation and Fractionation of Xenopus laevis Egg Extracts. J. Vis. Exp. (18), e891, doi:10.3791/891 (2008).More

Cross, M. K., Powers, M. Preparation and Fractionation of Xenopus laevis Egg Extracts. J. Vis. Exp. (18), e891, doi:10.3791/891 (2008).

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