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Preparation and Fractionation of Xenopus laevis Egg Extracts

Preparation and Fractionation of Xenopus laevis Egg Extracts

Article DOI: 10.3791/891-v 07:45 min August 27th, 2008
August 27th, 2008



Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.


Preparation Fractionation Xenopus Laevis Egg Extracts Crude Extract Fractionated Extracts Reconstituting Cellular Processes Morphological Analysis Biochemical Analysis Egg Lysis Differential Centrifugation Light Membrane Preparations
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