Mouse and rat pups are both most commonly used to study neonatal lung disorder. Due to the size of the pups, it is difficult to provide treatment (or study physiological changes) of these animals. Several methods have been described in the relevant literature, however, the inability to visualize these procedures has prohibited new investigators from attempting to adopt them into their work. This has led to a great deal of frustration: sharing those methods in a video format will not only help attract young researchers to join in the related research, but it will also increase the reproducibility and validation of the research results. Stem cell therapy, angiogenesis, lung function, cell isolation, and lung morphometry are a few important topics in studying neonatal lung growth. The proposed methods to be collected in this series include: (1) barium or microfilm angiography in young pups, (2) intratracheal injection of therapeutic agents or cells, (3) whole-body plethysmography for lung function, (4) alveolar cell isolation from young pups, and (5) designed based stereology for lung morphometry.
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Cited by 18
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2020
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Cited by 1
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2025
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1Department of Animal Sciences, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, 2Undergraduate Program in Biochemistry, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, 3Rutgers Center for Lipid Research, 4Rutgers Center for Environmental Exposures & Disease
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Cited by 1
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2021
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Emrah Aydın1,2, Rashika Joshi3, Marc Oria1, Foong-Yen Lim1, Brian Michael Varisco3, Jose Luis Peiro1
1The Center for Fetal, Cellular & Molecular Therapy, Cincinnati Fetal Center, Division of Pediatric General and Thoracic Surgery, Cincinnati Children’s Hospital Medical Center, 2Department of Pediatric Surgery, Tekirdag Namik Kemal University School of Medicine, 3The Division of Critical Care Medicine, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center
Induction of Lung Injury by oropharyngeal aspiration method
Rayees Sheikh*1,
Tusharika Kotra1,
Rahila Akhter1,
Yassir Arfath2,
Sheikh Tasduq Abdullah2,
Zabeer Ahmed*2
1CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu-180001 ,
2CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu-180001
Intratracheal Instillation of Stem Cells in Term Neonatal Rats
Yue-Jun Chen1,
Chung-Ming Chen*1
1Department of Pediatrics, School of Medicine, College of Medicine, Taipei Medical University