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Methods in Studying Lung Development in Mice and Rat Pups

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Ru-Jeng Teng

Ru-Jeng Teng

Medical College of Wisconsin

Dr. Teng graduated in 1985 from Medical College, National Taiwan University, and worked as a faculty neonatologist after his training since 1992 at National Taiwan University Hospital. He received his pediatric and neonatology training again in the US between 1997 and 2002. After a short career at the University of Florida, he joined the Medical College of Wisconsin as a faculty neonatologist since 2006. Throughout his career, he realized that I know so little about the pathophysiology behind most of the neonatal diseases. His present job provides him an opportunity to study the mechanisms of neonatal diseases that have the potential to help his patients. His initial research work was more clinical oriented and later switched to bench research after 2006. With the help of his collaborators, his research involves free radical biology, cell signaling, autophagy, and vascular biology. His utmost goal is to find treatments that can either attenuate the severity of bronchopulmonary dysplasia or facilitate the recovery from it. The most difficult problem he is facing during his work is to know how to adequately conduct an experiment described in the literature. This is especially true when trying to use small animals for their research. He believes this is also a major hurdle for new investigators who dedicate their time for similar work. To obtain a video version of the study method will benefit all that are willing to dedicate their work to provide a better outcome of prematurely born infants. 

Collection Overview

Mouse and rat pups are both most commonly used to study neonatal lung disorder. Due to the size of the pups, it is difficult to provide treatment (or study physiological changes) of these animals. Several methods have been described in the relevant literature, however, the inability to visualize these procedures has prohibited new investigators from attempting to adopt them into their work. This has led to a great deal of frustration: sharing those methods in a video format will not only help attract young researchers to join in the related research, but it will also increase the reproducibility and validation of the research results. Stem cell therapy, angiogenesis, lung function, cell isolation, and lung morphometry are a few important topics in studying neonatal lung growth. The proposed methods to be collected in this series include: (1) barium or microfilm angiography in young pups, (2) intratracheal injection of therapeutic agents or cells, (3) whole-body plethysmography for lung function, (4) alveolar cell isolation from young pups, and (5) designed based stereology for lung morphometry.  

 

 

Articles

Intratracheal Instillation of Stem Cells in Term Neonatal Rats
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Intratracheal Instillation of Stem Cells in Term Neonatal Rats

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Cited by 8

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2020

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Abstracts

Induction of Lung Injury by oropharyngeal aspiration method

Rayees Sheikh*1,

Tusharika Kotra1,

Rahila Akhter1,

Yassir Arfath2,

Sheikh Tasduq Abdullah2,

Zabeer Ahmed*2

1CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu-180001 ,

2CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu-180001

Intratracheal Instillation of Stem Cells in Term Neonatal Rats

Yue-Jun Chen1,

Chung-Ming Chen*1

1Department of Pediatrics, School of Medicine, College of Medicine, Taipei Medical University