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TOPICAL COLLECTIONS

Studying Resolution of Inflammation: Advances on Investigations on Cell Players, Endogenous Chemical Mediators, and Molecular Pathways

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Antonio Recchiuti

Antonio Recchiuti

University of Chieti, Center for Advanced Studies and Technology, Department of Medical, Oral, and Biotechnological Science

Dr. Antonio Recchiuti is Assistant Professor in Clinical Pathology at the Department of Medical, Oral, and Biotechnological Science and at the Center for Advanced Studies and Technology, University of Chieti, Italy. Dr. Recchiuti earned his MS Degree in Pharmaceutical Chemistry and Technology and his PhD at the University of Chieti. He completed his postdoctoral training at the Brigham and Women’s Hospital – Harvard Medical school with Charles N Serhan, who discovered endogenous pro-resolving lipid mediators (SPM) resolvins, lipoxins, protectins, and maresins that are critical in the resolution of inflammation. As a postdoc, Dr. Recchiuti identified the receptors binding the endogenous SPM resolvin D1, his signaling, and modes of action in human and mouse systems (PNAS 2010, FASEB J 2011, Am J Pathol 2012). He then moved back to the University of Chieti, where he has been a Group Leader since 2014.  The broad mission of his research team is to define bioactivities of SPM within the inflammatory and immune response and to promote the development of innovative therapies for the treatment of inflammation-based human pathologies, including cystic fibrosis and sickle cell disease. He has received several awards, including the ERC Marie Curie Grant, the Novartis Prize for Young Pharmacologists, and the Brigham and Women’s Hospital Spring Research Excellence Award. His research is funded by grants from the Italian Cystic Fibrosis Foundation, the American Cystic Fibrosis Foundation, the Telethon Foundation, and the Italian Ministry of Health. Link to publications: https://orcid.org/0000-0002-1409-5261  

Domenico Mattoscio

Domenico Mattoscio

University of Chieti, Center for Advanced Studies and Technology, Department of Medical, Oral, and Biotechnological Science

Domenico Mattoscio is Assistant Professor at the Department of Medical, Oral, and Biotechnological Science and at the Center for Advanced Studies and Technology, University of Chieti, Italy. Dr. Mattoscio received an MSc degree in Biology from the University of Molise, Italy, and then the PhD from the University of Chieti where he characterized the contribution of platelets to sustain chronic inflammation and failure in resolution in cystic fibrosis. He then moved to the European Institute of Oncology, Italy, where he dissected cellular pathways involved in viral-mediated tumorigenesis. After completion of his post-doctoral training, he moved back to the University of Chieti where his primary research interest is focused on the understanding of how the restoration of pro-resolution circuits is beneficial in chronic inflammatory disorders such as cystic fibrosis and cancer. The lab uses mouse models of disease coupled with multi-color flow cytometry, cell sorting, multiplex ELISA and RNA sequencing to detail the inflammatory response as a whole.

Collection Overview

Acute inflammation is a protective host response initiated following injuries or infections. In health, this life-saving reaction, normally proceeds towards its ideal outcome, resolution, with the removal of the inciting stimulus, the counterbalance of further leukocyte infiltration, and the repair of damaged tissue. If uncontrolled or unresolved acute inflammation can contribute to the pathophysiology of many recurrent human diseases.

The COVID-19 pandemic has made starkly clear that our understanding of mechanisms that regulate acute inflammation in the human body is still incomplete and that this knowledge gap hinders the possibility of treating inflammation-based pathologies in a more efficient manner.

Contrarily to previous thoughts, it is clear now that resolution of inflammation is an active process where several cell types, endogenous chemical mediators, and biological pathways are timely and tightly regulated.

Investigations in this field now can benefit from technological advances in cell biology, omics sciences, and immunology. This methods collection aims to assemble top researchers to share unique methods to explore the resolution of inflammation and exploit it therapeutically for many human diseases.


This Methods Collection invites protocols aimed to detail the resolution of inflammation as a whole, including but not limited to:

  1. Mouse models of disease;
  2. 3D culture methods to analyze the interplay between inflammatory and resident cells;
  3. Lipid and cytokine measurement;
  4. Imaging, flow cytometry, and cell sorting techniques;
  5. Omics approaches to characterize gene and protein expression levels.

Articles

Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples
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Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

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Cited by 3

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2023

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