Neurodevelopmental disorders affecting brain development and function are characterized by wide genetic and  variability. For example, different abnormalities in chromosome 15q11-13 regions have been proved to cause Angelman Syndrome, Prader-Willi Syndrome, and Autism. Exploring the underpinnings of such disorders (but not limited) by cutting edge methods releases the burden on patients and society in the long term but also broadens our understanding of ourselves. Indeed, the significant progress obtained from the research in in vitro studies (including cell lines and human-induced pluripotent stem cells (iPSC)) and in vivo explorations (such as Drosophila, Caenorhabditis elegans, rodents, and non-human primates) has pushed a big step towards clinic therapeutics. This collection of methods will include well-established cutting-edge techniques in the neurodevelopmental disorders field from in vitro to in vivo and from single-cell organisms to mammals. The establishment and popularization of these methods will further speed up our understanding of neurodevelopmental disorders and drug development.