The study of thrombosis and hemostasis has moved through many stages. Clinically, the pathogenesis of thrombus formation can be both an acute and a chronic process in the natural condition. However, direct experimental observation of this process in both in vitro and in vivo experiential models requires artificial methods. Based on the physiologic mechanisms of thrombus formation, each method has its advantages and disadvantages. Thus, familiarizing ourselves with the existing models and their pitfalls leads us to identify appropriate models to validate the study hypothesis and develop or improvise existing models to mimic human disease pathology.

This collection aims to establish a resource hub for researchers actively working in the thrombosis and hemostasis field as well as helps young investigators to perform the models in their studies with internationally accepted standard procedure to avoid experimental variations and reproducibility. This issue includes detailed protocols for in vitro, ex-vivo, and in vivo arterial and venous thrombosis models, protocols for bleeding models and fibrinolysis models, protocols for in vitro and in vivo imaging models, such as intravital microscopy to visualize platelet/leukocyte-endothelium interactions, Doppler ultrasound models to visualize thrombus development and resolution, and flow-based assays. Besides, this methods collection will also accept manuscripts from topics such as a novel in vitro and in vivo models.