
Artur De Castro Neto
Federal University of São Paulo, Paulista Medical School, Department of Microbiology, Parasitology and Immunology, Brazil
Dr. Artur de Castro Neto has been working with Trypanosomatids for 11 years. He received his master’s degree in 2014 and PhD in 2018 from the Department of Genetics at the Federal University of Pernambuco, Brazil. There he worked in the laboratory of Dr. Osvaldo de Melo Neto at FIOCRUZ, where his master’s research led to the development of a novel visceral leishmaniasis diagnostic test, fit to humans and dogs. His thesis shed light on genomic events that might have led to Leishmania virulence proteins expansion. In 2018, he started his post-doctoral research in the laboratory of Prof. Sue Vaughan in the Department of Biological and Medical Sciences at Oxford Brookes University in Oxford, UK, where he worked with structural biology of proteins involved in basal body biogenesis and flagellum formation in Trypanosoma brucei. He is currently a post-doctoral fellow in the Department of Microbiology, Immunology and Parasitology at Paulista Medical School from the Federal University of São Paulo, Brazil in the laboratory of Prof. Renato Mortara. His research is focused on assessing components released by different strains of Trypanosoma cruzi and their role in the parasite’s virulence.

Eden Ramalho Ferreira
The University of York, York Biomedical Research Institute, Department of Biology, Wentworth Way, Heslington, York, UK
Dr. Eden is a post-doctoral research associate at the University of York working with Prof. Jeremy Mottram. He obtained his BSc in Biology and his MSc in Sciences with emphasis on Cell Biology and Parasitology. In 2016 he obtained his PhD degree at the Federal University of Sao Paulo working at Prof. Renato Mortara´s lab, investigating the role of host-cell cytoskeleton associated proteins during invasion of extracellular amastigotes of Trypanosoma cruzi. From the year 2017 to 2021 he worked as post-doctoral research fellow funded by The Sao Paulo Research Foundation investigating how trypomastigotes of T. cruzi manages its escape from the host cell. During this period, he spent 12 months at Prof. Kevin Tyler’s lab – University of East Anglia (Norwich – UK) as a visiting researcher funded by the Sao Paulo Research Foundation for an abroad internship. Currently, he is working on the Wellcome trust funded grant – LeishGEM. His primary focus is the subcellular localization of proteins from Leishmania mexicana. In addition, he is also working with genetic modified cell lines for the identification of molecules important for the interaction of Leishmania parasite with its host.
Trypanosoma cruzi, Trypanosoma brucei, and Leishmania spp., are flagellate protozoans of the family Trypanosomatidae and the causal agents of human African trypanosomiasis, Chagas disease, and different forms of leishmaniasis, respectively. These diseases affect millions of humans worldwide, mainly in tropical and subtropical countries, and exert a significant impact on public health. To control these pathologies, many studies have been done seeking to better understand their biology with the goal to promote advances in solving or mitigating these health issues. These studies include the improvement of diagnostic methods, development of vaccines, and novel drug treatment alternatives. In addition, these trypanosomatids have also been extensively used as a model for eukaryotic cell studies, due to their conservation relative to molecules, machinery, processes, and organelles. To do that, molecular and cell biology techniques have been used and are in constant progress to develop novel approaches to the control of these diseases and to a better understanding of the parasite biology and disease progression. The aim of this Methods Collection is to display current molecular and cellular biology standardized methods for both applied and basic research in trypanosomatids.
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2023
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Jafeth Carrasco*1, Jim H. Chang*1, Laura Pineda1, Iliana Quintero1, Rita Giovani2, Carmenza Spadafora1,3, Ricardo Lleonart1,3, Carlos M. Restrepo1,3
1Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), 2Departamento de Medios y Creativo, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), 3Sistema Nacional de Investigación-Secretaría Nacional de Ciencia, Tecnología e Innovación (SNI-SENACYT)
An agarose-microwell approach for trypanosomatid live-cell imaging
Christopher de Graffenried*1
1Brown University
High throughput in vitro assay for antileishmanial compound screening based on eGFP-expressing Leishmania panamensis parasites
Carlos Restrepo*1
1INDICASAT AIP