Brucellosis remains a significant zoonotic disease globally. With well-developed veterinary and laboratory infrastructure, sufficient resources, and political will it is possible to control, and ultimately even eradicate, brucellosis. However, it remains endemic in many low- and middle-income countries. Furthermore, without vaccines suitable for human use, the control of human disease is dependent upon reducing animal-derived risks and ultimately controlling the animal disease. Many techniques are applied to the diagnosis of infection and characterization of the agent; these include serological and bacteriological approaches, many of which have been applied for decades, but are being increasingly supplemented by newly developed molecular approaches. Unfortunately, where both classical and emerging approaches are most needed, access to external training, quality assessment or other means to ensure they are being undertaken optimally is often most difficult. Hence the visual methods collection proposed here will be of great value in the toolbox of researchers attempting to tackle brucellosis. Furthermore, it is intended that this collection include not only methods applied to diagnosis and pathogen characterization, but also emerging techniques used in cutting edge research in order to understand the fundamental biology of the pathogen. The objective of this methods collection is to provide a platform with which to share standard approaches, promoting better access to existing and emerging methods globally. Such a comprehensive collection will be invaluable to the broad brucellosis community, from those involved in diagnostic work in laboratories with local, national and international responsibilities to research laboratories exploring fundamental or applied aspects of Brucella and brucellosis.