Collection-image

TOPICAL COLLECTIONS

Methods for Examining the Hallmarks of Cancer
Submit Abstract

Guest Editors

Stephany Veuger

Stephany Veuger

Northumbria University

A significant focus of Stephany Veuger's research has been to evaluate DNA repair inhibitors as potential drugs to be used in combination with current therapies or as stand-alone therapies in cancer treatment. She has supported anticancer drug discovery projects by (1) evaluating drugs to exploit underlying molecular defects in a range of human cancers,(2) investigating interactive effects of proteins involved in sensing and responding to DNA damage,(3) developing and validating pharmacodynamic biomarkers for agents progressing to clinical trials.She has played a vital role in the drug development group at the Northern Cancer Centre. Her work evaluating agents progressing to clinical trials and developing biomarkers contributed to the successful introduction of PARP inhibitors into patients. She is interested in solid tumors (breast and prostate) and hematological malignancies. She currently collaborates with medicinal chemists to explore the underlying biological mechanisms of novel therapeutics alone and in combination with repair inhibitors.  

Seth Racey

Seth Racey

Northumbria University

As a multi-disciplinary researcher, Dr. Racey has research outputs in molecular ecology, circadian clocks, orthopedic implants, and stem cells for regenerative medicine. His research recently focuses on using iron ion manipulation with novel metal chelators to treat cancers. He also has the background to recruit collaborators from disciplines that can inform cancer biology and bring novel perspectives. 

Gina Abdelaal

Gina Abdelaal

Northumbria University

Gina Abdelaal is a PhD student at Northumbria University supervised by Dr. Stephany Veuger. Her research project involves screening a panel of cell lines for their sensitivity to in-house synthesized novel iron chelator SK4 and determining the mechanism behind SK4 action. She is experienced in cell culture methodology. She has grown and screened ten different cell lines during her PhD and optimized various laboratory techniques related to the hallmarks of cancer (cell viability assays, flow cytometry, Western blotting, PCR, immunofluorescence, and proteomics). She is also a peer reviewer for STAR protocols and evaluates new methods papers for their clarity, content, and ease of reproducibility. 

Collection Overview

Since the initial publication by Hanahan and Weinberg in 2000, which sought to develop a set of organizing principles to stratify and distill the daunting multiplicity of cancer into a framework of characteristics, the hallmarks of cancer have evolved, and so have the methods used to support cancer research. There are many existing and validated techniques to measure features, including proliferation and cell death. The expanding picture of cancer biology to include reprogramming of energy metabolism, avoiding immune destruction, tumor-promoting inflammation, and evading immune destruction has necessitated the need for novel methods and assays to support the further understanding of cancer pathogenesis and ultimately pave the way for novel cancer therapies. 

Each hallmark possesses characteristics that can be analyzed by a variety of means. In this Methods Collection, we bring together a wide range of methods, including novel techniques capable of determining the characteristics of each hallmark, including those that allow simultaneous evaluation of multiple hallmarks. We will include relevant markers and seek to showcase methods that assess more complex but less well-characterized features, such as altered energy metabolism beyond glucose and oxygen measurements through measures of epigenetics and the discovery and validation of marker genes. 

Articles

Quantifiable and Inexpensive Cell-Free Fluorescent Method to Confirm the Ability of Novel Compounds to Chelate Iron
5:36

Quantifiable and Inexpensive Cell-Free Fluorescent Method to Confirm the Ability of Novel Compounds to Chelate Iron

0 Views

•

Cited by 1

•

2024

•

Abstracts

MS-based profiling of histone marks for epigenetic mapping of cancer patient samples

Giulia Robusti1,

Paola Fulghieri2,

Tiziana Bonaldi*2

1Department of Experimental Oncology, IEO, European Institute of Oncology IRCCS, Milan, Italy,

2Department of Experimental Oncology, IEO, European Institute of Oncology IRCCS, Milan, Italy; Department of Oncology and Hematology-Oncology (DIPO), University of Milan, Milan, Italy