Exploring the vascularization of an organ or tissue is a key factor in understanding its metabolism, its development, and its potential pathological features. Nevertheless, the analysis of the "vascular tree" has long been restricted to the visualization of the largest arterial trunks. The development of bioengineering and imaging allows us to propose new approaches to visualize the microvascular network and quantify perfusion. These technologies must be evaluated and validated by in vivo, ex vivo, and in vitro analyses, which is particularly challenging due to the small size of these vessels.

This Methods Collection aims to bring together articles on innovative methodologies in imaging and bioengineering to provide methods for imaging evaluation and to encourage translational research projects toward clinical imaging and in vivo vasculature quantification, as well as to encourage other researchers and clinicians to adapt and expand on these techniques to their specific needs.