Macrophages and monocytes are central regulators of innate immunity, tissue homeostasis, and inflammatory processes, and their dysfunction is implicated in a wide range of diseases, including infections, cancer, and autoimmune disorders. Understanding their biology is critical for both fundamental research and the development of novel therapeutic strategies.

Despite extensive study, challenges remain in reliably isolating these cells, maintaining their physiological states, manipulating them without altering their natural behavior, and accurately characterizing their dynamic functional and phenotypic profiles. Variability in experimental approaches and the complexity of these populations often limit reproducibility and comparability across studies.

This Collection aims to provide a comprehensive platform for diverse approaches to study macrophages and monocytes. It will highlight methods that enable reliable isolation, manipulation, and characterization while preserving cellular integrity and functional relevance. The collection seeks to include techniques suitable for controlled experimental settings as well as approaches that maintain close relevance to in vivo conditions, fostering reproducible and physiologically meaningful investigations.

By consolidating these methodologies, the collection will serve as a valuable resource for immunologists, cell biologists, and translational researchers, supporting high-quality, reproducible studies. It will advance understanding of macrophage and monocyte biology, promote standardized approaches in the field, and facilitate the discovery of novel therapeutic targets, ultimately bridging fundamental research with clinical applications.