Mitochondria form a dynamic, interconnected network within the cell. The term "mitochondrial dynamics" encompasses the processes of fission, fusion, mitophagy, and trafficking. This dynamic rearrangement of the mitochondrial complex reticulum responds to factors such as the nutritional environment, biological signals, mechanical forces, and cellular stress.

These mechanisms are essential for optimal cellular signal transduction and metabolism. Mitochondrial dysfunctions can be caused by an imbalance in mitochondrial dynamics, leading to abnormal cellular outcomes and a spectrum of pathological conditions, including neurodegenerative, metabolic, and cardiovascular disorders, cancers, and, more in general, human aging.

Comprehending the molecular processes governing mitochondrial dynamics is essential. It is crucial to use appropriate strategies and techniques to analyze how mitochondrial shape affects functions and processes. This contributes to our understanding of the molecular basis of diseases associated with defects in mitochondrial morphology.

This Methods Collection is dedicated to elucidating pertinent laboratory approaches that advance scientific research and teaching. It focuses on procedures, methods, techniques, and analyses employed to investigate mitochondrial dynamics and aims to enhance knowledge in this field