Neurodevelopmental disorders encompass a broad range of diseases, including epilepsy, autism spectrum disorder (ASD), intellectual disability, and other CNS abnormalities. This Methods Collection will highlight the most advanced methodologies for studying neurodevelopmental disorders. Techniques may include AI assessment of neural tube defects, assembloid models to study cortical malformations, in utero electroporation in ferrets or mice to study focal malformations, prime-editing, spatial transcriptomics, high-throughput zebrafish models, and other bench-based and computational methods. These techniques have a wide range of applications beyond the field of neurodevelopmental disorders and cortical malformations, making them of interest to others in the neuroscience community.