Research in animal regeneration offers critical insights into developmental biology, evolution, and the fundamental mechanisms that enable tissue and organ renewal. It also holds immense promise for advancing regenerative medicine, including wound healing and organ repair. Recent advances, particularly in molecular techniques for axolotls and zebrafish, have led to innovative new tools that can provide researchers with unprecedented opportunities to investigate the genetic and cellular mechanisms underlying regeneration. This collection highlights modern research methods for studying regenerative processes in both model and non-model organisms. This will broaden access to the techniques of the field, enabling a wider range of researchers in regenerative biology to employ these tools.