This JoVE Methods Collection focuses on advanced protocols in stem cell biology and regenerative medicine, organized to accelerate therapeutic advances. As regenerative therapies transition from the bench to the bedside, reproducible, visually detailed methods are crucial for advancing stem cell isolation, expansion, differentiation, and application in tissue repair. Contributions will feature innovative techniques, including pluripotent stem cell reprogramming, mesenchymal stem cell characterization and immunomodulation, directed differentiation into lineage-specific progenitors, 3D organoid modeling for disease and regeneration studies, genome editing, bioengineered scaffolds for stem cell delivery, and preclinical models for evaluating therapeutic efficacy in injury and degenerative diseases.

The collection aims to provide high-quality, peer-reviewed video protocols that visually demonstrate cutting-edge techniques in stem cell isolation, characterization, engineering, and application. By offering step-by-step visual guidance, it will enhance experimental reproducibility, promote methodological standardization across laboratories, lower barriers for researchers entering the field, and ultimately accelerate the development of robust, clinically viable regenerative therapies. This resource will empower the global research community to advance both fundamental discoveries and translational innovations in regenerative medicine.