The exploration of biomolecular interactions is crucial for a better understanding of the fundamental functions and mechanisms that govern biological processes. In a non-invasive manner, fluorescence based methods enable real-time detection of processes that can occur intracellularly or extracellularly and therefore have been widely used to study molecular interactions in both basic and applied research. Over the last decades, development of fluorescence techniques using both endogenous and exogenous probes has provided appropriate tools to understand the nature of a wide range of biomolecular interactions. In addition, brighter photostable dyes and new microscopy technologies have highly contributed to the development of fluorescence imaging.

The goal of this collection is to bring together different methods based on fluorescence properties and to combine both classical techniques such as fluorescence spectroscopy and new innovative technologies such as single-particle tracking and super-resolution microscopy. The collection will include different types of biomolecular interactions (protein-protein, protein-membrane, protein-nucleic acid) with an interest in, but not limited to, biological processes that occur at or near the cell membranes. Recent developments in membrane models or molecular biology tools used for fluorescence applications may be included as well.