
Since the emergence of Direct Electron Detectors in 2014, cryo-EM has transformed structural biology, and is rapidly becoming the leading method for protein structure determination, both in academia and industry. Importantly, the pace of method development has increased steadily since then, with new ways to handle samples, and collect and process data being reported on a regular basis. In particular, recent developments towards the study of proteins smaller than 100 kDa, within cells, or up to atomic resolution highlight the still-expanding potential of cryo-EM, and the need for greater reproducibility. This Method Collection brings together recent developments in grid preparation, data collection, and processing for single-particle analysis, but also cryo-electron tomography, 2D-crystallography and micro-ED.
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2021
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1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 3Department of Physiological Sciences, Eastern Virginia Medical School
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Cited by 8
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2021
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Cited by 8
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2021
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Claudine Bisson1,2, Corey W. Hecksel3,4, James B. Gilchrist3, M. Alejandra Carbajal1, Roland A. Fleck1
1Centre for Ultrastructural Imaging, New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science, Birkbeck College, University of London, 3Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory, Stanford University
Cryo-EM grid preparation for SARS-CoV-2 spike ectodomain and HIV-1 Env samples
Priyamvada Acharya*1
1Duke University