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TOPICAL COLLECTIONS

Recent Advances and Best Practices in Cryo-EM Analysis for Protein Structure Determination.

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Guest Editor

Julien Bergeron

Julien Bergeron

King’s College-London, Randall Division of Cell and Molecular Biophysics

Julien Bergeron is a Senior Lecturer in Electron Microscopy, in the Randall Centre for Cell and Molecular Biophysics, King’s College London. His research research program focuses on using cryo-EM, combined with other structural and biophysical methods (X-ray crystallography, NMR, molecular modelling, ITC etc…), to decipher the structure of macromolecular nano-machines, focusing mainly on four themes: bacterial motility and adherence; macromolecular transport across the bacterial membranes; the bacterial cytoskeleton and microcompartments; and host-viral interactions. Julien received his PhD from KCL in 2010, where his research aimed at using NMR to study the recruitment of a ubiquitin ligase complex by the HIV protein Vif. Subsequently, he took post-doctoral fellow positions at the University of British Columbia (Vancouver, Canada) and the University of Washington (Seattle), where he studied large bacterial complexes such as the type III secretion system and the bacterial cytoskeleton, by X-ray crystallography and cryo-EM. He established his group at the University of Sheffield (UK) in 2017, and returned to KCL two years later.

Collection Overview

Since the emergence of Direct Electron Detectors in 2014, cryo-EM has transformed structural biology, and is rapidly becoming the leading method for protein structure determination, both in academia and industry. Importantly, the pace of method development has increased steadily since then, with new ways to handle samples, and collect and process data being reported on a regular basis. In particular, recent developments towards the study of proteins smaller than 100 kDa, within cells, or up to atomic resolution highlight the still-expanding potential of cryo-EM, and the need for greater reproducibility. This Method Collection brings together recent developments in grid preparation, data collection, and processing for single-particle analysis, but also cryo-electron tomography, 2D-crystallography and micro-ED.

Editorial

Recent Advances and Best Practices for Cryo-EM Analysis for Protein Structure Determination

Recent Advances and Best Practices for Cryo-EM Analysis for Protein Structure Determination

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2023

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Articles

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
10:05

Abstracts

Cryo-EM grid preparation for SARS-CoV-2 spike ectodomain and HIV-1 Env samples

Priyamvada Acharya*1

1Duke University